The Effects of Fuzheng Huayu Recipe on Collagen Ⅳ Expression and MMP-2 Activity in Rat Fibrotic Liver

CUI Hong-yan1; 2; JIANG Zhe-hao1; LU Xiong3; LIU Cheng1; LIU Cheng-hai1

您当前的位置：

首页 >

文章列表页 >

The Effects of Fuzheng Huayu Recipe on Collagen Ⅳ Expression and MMP-2 Activity in Rat Fibrotic Liver

更新时间：2024-01-04

- The Effects of Fuzheng Huayu Recipe on Collagen Ⅳ Expression and MMP-2 Activity in Rat Fibrotic Liver
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 7, Pages: 43-47(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2008
- 稿件说明：
移动端阅览
CUI Hong-yan1, 2, JIANG Zhe-hao1, et al. The Effects of Fuzheng Huayu Recipe on Collagen Ⅳ Expression and MMP-2 Activity in Rat Fibrotic Liver[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 43-47.
DOI：

CUI Hong-yan1, 2, JIANG Zhe-hao1, et al. The Effects of Fuzheng Huayu Recipe on Collagen Ⅳ Expression and MMP-2 Activity in Rat Fibrotic Liver[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 43-47. DOI：

摘要

目的:探讨扶正化瘀方影响基质金属蛋白酶-2活性和Ⅳ型胶原蛋白表达的抗肝纤维化作用机制。方法:二甲基亚硝胺腹腔注射4周诱导大鼠肝纤维化模型

成模后治疗组以扶正化瘀方灌胃4周。检测血清肝功能、肝脏病理学、羟脯氨酸含量;免疫组织化学方法检测肝组织Ⅳ型胶原表达;Western印迹法分析肝组织Ⅳ型胶原、基质金属蛋白酶-2/9(matrix-metalloproteinases-2

MMP-2/9)、金属蛋白酶组织抑制物-2(tissue inhibitors of metalloproteinase-2

TIMP-2)表达水平;明胶酶图法检测肝组织MMP-2/9活性。结果:与正常大鼠比较

模型大鼠血清肝功能异常、肝组织炎症明显、肝组织Ⅳ型胶原表达与沉积增加

TIMP-2蛋白表达增加

MMP-2蛋白表达变化不明显

而MMP-2活性水平明显上升。扶正化瘀方显著减轻肝脏炎症和胶原沉积;减少Ⅳ型胶原蛋白表达和沉积;降低TIMP-2蛋白表达;抑制MMP-2活性。结论:扶正化瘀方可通过抑制肝脏Ⅳ型胶原病理增生沉积、下调MMP-2活性水平、减轻肝组织的破坏与重构而发挥抗肝纤维化的作用。

Abstract

Objective: To investigate the effects of Fuzheng Huayu recipe(FZHY recipe) on collagen Ⅳ expression and matrixmetalloproteinases-2(MMP-2) activity

explore the mechanisms of the recipe action against liver fibrosis.Methods: Liver fibrosis was induced by intraperitoneal injection of dimethylnitrosamine(DMN).The rats in FZHY group were administered with FZHY recipe ig for 4 weeks after the model was established.Serum ALT

AST activities

total bilirubin and Alb levels were determined using commercial kits;serum hepatic inflammation and collagen deposition were evaluated with HE staining and Sirius-red staining respectively;the contents of hepatic hydroxyproline(Hyp) were measured by Jamall's method.expression of collagen Ⅳ in liver tissue was analyzed by immunohistochemistry;the protein expressions of collagen Ⅳ

MMP-2

MMP-9 and TIMP-2 were analyzed by Western blotting;and the activities of MMP-2/9 were analyzed by gelatin zymography.Results:Abnormal liver function

obvious hepatic inflammation

excess collagen deposition

high protein expressions of collagen Ⅳ and TIMP-2

and increased activities of MMP-2 were observed in model group

compared with those in normal group.Compared to model group

FZHY recipe significantly alleviated liver inflammation and the excess collagen deposition;Also it significantly down-regulated protein expression of collagen Ⅳ and TIMP-2

and inhibited the activity of MMP-2.Conclusion: The mechanisms of FZHY recipe action against liver fibrosis were related to inhibiting the excessive deposition of collagen Ⅳ and down-regulating MMP-2 activity in liver tissue.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism and Combination Therapy of Berberine in Treatment of Nonalcoholic Fatty liver Disease：A Review

Clinical Efficacy of Gandou Fumu Granules in Intervention of Liver Fibrosis in WD Patients with Phlegm and Blood Stasis Syndrome and Effect on Cuproptosis-related Indicators

Gandouling Regulates PI3K/Akt/mTOR Autophagy Signaling Pathway via LncRNA H19 for Treatment of Wilson Disease Liver Fibrosis

Mechanism of Shugan Huazheng Prescription Against Liver Fibrosis Based on HIF-1α/VEGF/TGF-β₁ Pathway

Effect of Gandou Fumu Decoction on Autophagy in Mice with Liver Fibrosis in Wilson's Disease by Regulating Expression of miR-29b-3p/ULK1

Related Author

WANG Xiaojie

ZHANG Heng

LIU Sutong

ZHANG Lihui

ZHAO Wenxia

WANG Fei

JIANG Zhenzhen

CHEN Yimin

Related Institution

Henan General Hospital

The First Affiliated Hospital of Henan University of Chinese Medicine

The First Clinical Medical College of Henan University of Chinese Medicine

The First Affiliated Hospital of Anhui University of Chinese Medicine

Graduate School of Anhui University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰