Influence of Total Flavones of Hippophae Rhamnoides L on Secretion of t-PA,PAI-1,vWF from Endothelial Cells

CHENG Jia-yi; TENG Dan; SUN Jing; KANG Ting-guo

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Influence of Total Flavones of Hippophae Rhamnoides L on Secretion of t-PA,PAI-1,vWF from Endothelial Cells

更新时间：2024-01-04

- Influence of Total Flavones of Hippophae Rhamnoides L on Secretion of t-PA,PAI-1,vWF from Endothelial Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 7, Pages: 38-40(2008)
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- Published：2008
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CHENG Jia-yi, TENG Dan, SUN Jing, et al. Influence of Total Flavones of Hippophae Rhamnoides L on Secretion of t-PA,PAI-1,vWF from Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 38-40.
DOI：

CHENG Jia-yi, TENG Dan, SUN Jing, et al. Influence of Total Flavones of Hippophae Rhamnoides L on Secretion of t-PA,PAI-1,vWF from Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 38-40. DOI：

摘要

目的:观察沙棘总黄酮对ECV-304细胞分泌组织型纤溶酶原激活物(t-PA)、纤溶酶原激活物抑制物-1(PAI-1)、血管性血友病因子(vWF)的影响并探讨其可能的活性成分。方法:以不同浓度的沙棘总黄酮和异鼠李素与培养的ECV-304细胞共同孵育18h后

用ELISA法检测培养液中t-PA

PAI-1

vWF的含量。结果:沙棘总黄酮和异鼠李素均可剂量依赖性抑制ECV-304细胞PAI-1的分泌

与空白对照组相比

两者高

中剂量组均有显著性差异(P<0.01)

低剂量组无显著性差异;沙棘总黄酮和异鼠李素对ECV-304细胞分泌t-PA和vWF均无明显影响。结论:沙棘总黄酮抑制内皮细胞PAI-1的分泌

异鼠李素可能是该作用的活性成分之一。

Abstract

Objective:To observe the influence of total flavones of Hippophae Rhamnoides L(TFH) on secretion of t-PA

PAI-1

vWF from ECV304 cells and identify the possible active component.Methods: Cultured ECV-304 cell was commonly hatched 18 hours with The TFH and isorhamnetin of different concentrations

then the contents of t-PA

PAI-1

vWF in culture fluid were determined by ELISA. Results: The TFH and isorhamnetin of different concentrations could inhibit secretion of PAI-1 from ECV-304 cells in a concentration-dependent manner.Compared with the control group

high and middle concentration groups significantly decreased the contents of PAI-1(P<0.01).TFH and isorhamnetin didn't influent the secretion of t-PA and vWF.Conclusions:TFH could inhibit PAI-1 secretion of endothelial cell

isorhamnetin may be one of the active components.

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Related Institution

The Second School of Clinical Medicine，Zhejiang Chinese Medical University

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The Affiliated Hospital of Guizhou Medical University

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