Objective:To observe the dynamic interference in LBP mRNA

CD14 mRNA

NF-κBp65 on the cell endotoxin(ET) specific receptors of damp-heat syndrome model rats treated by Ganlu Xiaodu Dan

furthe to explore the therapy mechanism of Qingre Zaoshi Prescription.Methods: Animal were divided into normal group

model group(high fat diet+high temperature and high humidity+colon bacillus)

Qingre Zaoshi group randomly

the expression of LBP mRNA

CD14 mRNA

in liver macrophage was semiquantitated by reverse transcription-polymerase chain reaction(RT-PCR)

the activation of nuclear factor-kappa Bp65(NF-κBp65) in liver macrophage was detected by immuno-histochemical methods.Results:At 6 h

12 h and 24 h action spots

the expressions of LBP mRNA

CD14 mRNA

NF-κBp65 in model group was remarkably increased compared with those of normal group;And at 6 h

12 h and 24 h

the above indicators had significant difference compared with each other.The result indicated that during the development of the course of disease

the expression of LBP mRNA

CD14 mRNA

NF-κBp65 in model group was increased gradually.The expression of LBP mRNA

CD14 mRNA

in treatment group was decreased

and had significant difference between 6 h and 12 h and 12 h and 24 h.At 24 h

the expression of LBP mRNA

CD14 mRNA in treatment group was decreased

and had significant difference compared with model group.The results suggested that attenuation of LBP mRNA

CD14 mRNA

is a slow course. Conclusions:It was suggested that Ganlu Xiaodu Dan has interference in LBP mRNA

CD14 mRNA

NF-κBp65 in liver macrophage of damp-heat syndrome of seasonal febrile disease rats.