Objective:To study the in vitro inhibitory of protoporphyrin disodium(PPN) on Hepatitis C virus.Methods:The different concentration of PPN and ribavirin water solution were respectively added into HCV Replicon cell medium.The cell toxicity of PPN was determined with MTT method.Real-time PCR was used for determining secrected HCV RNA in the culture medium.Results:PPN and ribavirin had no obvious cell toxicity at the concentration of 1 mg/mL.When the drug concentration of PPN was 1 mg/mL

inhibitive rates of HCV RNA was 73.44%.The experimental group and control group had striking difference(P<0.05)

When the drug concentration of ribavirin was 1 mg/mL

inhibitive rates of HCV RNA was 40.78%.EC50 were 0.23 mg·mL-1 and 1.19 mg·mL-1 respectively.Conclusion:PPN can inhibit duplication of Hepatitis C Virus in Vitro and its effect was better than ribavirin.