Objective:To study depressant efects of the extract of Radix Tetrastigma Hemsleyani on lung carcinoma cell line A549 in vitro.Methods:The time-effect and quality-effect of the extract of Radix Tetrastigma Hemsleyani on lung carcinoma cell line A549 were observed by cell culturein vitro using the method of MTT.Agarose gel electrophoresis was used to detect the apoptosis.RT-PCR were used to detect the expression of BcL2 gene

which was related to apoptosis.Results:48 hours after co-culturing A549 and Radix Tetrastigma Hemsleyani with a concentration of 1×10-3 g·L-1.the cell activity decreased significantly compared to control.When culturing A549 and the extract with different concentrations

it had a significant depressant effect on cell activity with concentrations of 1×10-2

1×10-3

1×10-4 g·L-1.There was an obvious apoptotic ladder in agarose gel electrophoresis.Culturing A549 and the extract with different concentrations

the expression of BcL-2 gene was low following the density raise of Radix Tetrastigma Hemsleyani.Conclusions:the extract of Radix Tetrastigma Hemsleyani has a significant depressant effect on lung carcinoma cell line A549

which positively relates with its concentration and efect-time.