Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats
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Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 14, Issue 5, Pages: 40-43(2008)
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Published:2008
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SU Jing-ze, NONG Yi-bing, WEN Zhi-hao, et al. Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(5): 40-43.
DOI:
SU Jing-ze, NONG Yi-bing, WEN Zhi-hao, et al. Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(5): 40-43.DOI:
Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats
Objective:To observe whether Radix Astragali saponins and Astragali polysaccharides can effectively interfere with the cellular energy metabolism and its mechanism and to provide scientific evidence for the application of the Chinese herbs preventing and treating congestive heart failure.Methods:mRNA expression of isoenzyme creatine kinase(iso-CK) was investigated by means of RT-PCR
based on the study of the effect of Radix Astragali saponins and Astragali polysaccharides
through the cultured neonatal myocyte hypetrophy induced by AngⅡ.Results: 1.The expression of CK-B subunits was increased significantly while the expression of CK-M subunits was decreased by AngⅡ since the 24th hours
at 48th hours
the expression still stay the same level.2.The expression of iso-CK: Subunits in Radix Astragali saponins and Astragali polysaccharides influenced kept it at the normal level at 48th hours.Conclusion: The influence of AngⅡ on the mRNA expression of isoenzyme creatine kinase can be effectively inhibited by Radix Astragali saponins and Astragali polysaccharides.Radix Astragali can prevent the development of heart failure
the possible mechanism is Radix Astragali interferes the myocyte hypetrophy in terms of the cellular energy metabolism.