Objective:To establish an analytical method of RP-HPLC-DAD to determinate simultaneously the chemical components of vanillic acid

caffeic acid

albiflorin

peoniflorin

ferulaic acid

isorhamnetin-3-O-rutinoside

isorhamnetin-3-O-neohesperidoside in Shaofu Zhuyu Decoction and to provide evidence for the control and elucidate the bioactive components of Shaofu Zhuyu Decoction.Methods:The method of RP-HPLC-DAD was adapted to determinate the seven compounds in the sample simultaneously.A Waters SunFire-C18 column(4.6 mm×250 mm

5 μm) was used with the mobile phase consisting of acetonitrile(A)

0.05% phosphoric acid(B) and methanol(C) at the flow rate of 1.0 mL·min

sup>-1 in gradient elution(0 min:8∶87∶5

30 min:13∶82∶5

35 min:13∶82∶5

55 min:14∶81∶5

60 min:14∶81∶5

80 min:15∶80∶5).The detected wave were 260

230

254

and 320 nm respectively and the temperature of column was 30℃.Results:The linearity of the 7 compounds was good

and the average recovery was range of 97.8%～100.2%.Conclusions:The method of HPLC-DAD is accurate and sensitive with good repeatability for the determination of 7 compounds simultaneously in Shaofu Zhuyu Decoction.The results provide references to analyze the complicated components of TCMs.