The Preparation of Active in vitro Membrane and Its Adsorption of Astragaloside IV and Ferulic Acid in Buyang Huanwu Decoction

LIU Wei1; HE Fu-yuan1; 3; LIU Wen-long1; WU Yong1; WANG Zhuxin2

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The Preparation of Active in vitro Membrane and Its Adsorption of Astragaloside IV and Ferulic Acid in Buyang Huanwu Decoction

更新时间：2024-01-04

- The Preparation of Active in vitro Membrane and Its Adsorption of Astragaloside IV and Ferulic Acid in Buyang Huanwu Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 2, Pages: 21-25(2008)
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- Published：2008
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LIU Wei1, HE Fu-yuan1, 3, et al. The Preparation of Active in vitro Membrane and Its Adsorption of Astragaloside IV and Ferulic Acid in Buyang Huanwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(2): 21-25.
DOI：

LIU Wei1, HE Fu-yuan1, 3, et al. The Preparation of Active in vitro Membrane and Its Adsorption of Astragaloside IV and Ferulic Acid in Buyang Huanwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(2): 21-25. DOI：

摘要

目的:研究体外细胞膜的制备工艺及其对中药复方补阳还五汤中黄芪甲苷及阿魏酸的吸附性。方法:采用蔗糖溶液分步离心法制备体外细胞膜

并按常规的柱层析分离补阳还五汤中的黄芪甲苷及阿魏酸

再用高效液相色谱法测定其含量变化。结果:用该法制得的细胞膜中蛋白质的含量约为8.0%±0.45%

黄芪甲苷的流出达峰时间为1h

浓度为123.6μg·mL-1

转移率为94.46%;阿魏酸的流出达峰时间为9h

浓度为8.769μg·mL-1

转移率为86.78%。结论:体外细胞膜对黄芪甲苷和阿魏酸的吸附选择性有显著性差异

可用于中药复方成分的分离。

Abstract

Objective:To study the isolation of the active in vitro cell membranes of pig intestines and adsorption of astragaloside IV and ferulic acid in Buyang Huanwu Decoction(BYHWD).Methods:The sucrose density gradient ultracentrifugection at different speeds was used to isolate pig intestinal membranes and determine it's total protein content.Normal column chromatography was used to isolate the astragaloside IV and ferulic acid

the content of which was determined by HPLC analysis.Results:The content of total protein in the cell membrane is 8.0%±0.45%

The peak time for astragaloside IV is 1 h

while the concentration is 123.6 μg·mL-1 and the transferring ratio is 94.46%;The peak time for ferulic acid is 9 h

while the concentration is 8.769 μg·mL-1 and the transferring ratio is 86.78%.Conclusions:There are significant difference in adsorbability and transporting capability between astragaloside IV and ferulic acid under cell membrane chromatography.The cell membrane could be used to separate the ingredients of the compound formulate of traditional Chinese medicine.

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Related Author

ZHANG Cui-ying1

LI Zhen-guo1

MA Xiao-feng2

XU Jin-ling1

LIU Nai-qiang1

LI Wan-qing

FAN Jun-ting

LIU Yong

Related Institution

Dept.of Pharmacy,General Hospital Group Oilfield Hospital of Daqing

Liaoning University of Traditional Chinese Medicine

Henan Hospital of Traditional Chinese Medicine

Henan College of Traditional Chinese Medicine

Department of Pharmacy,the Second People’s Hospital of Zhengzhou City

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