Objective:To explore the change of immunological system in model rat of spleen-qi deficiency induced by complex factors.Methods:Adopting excess fatigue (by swimming) and out of constant diet on rats for constant 3 weeks and stopping doing so in the 4 th week.We observed the change of the weight of thymus and spleen

the index of thymus and spleen

and cell factors of serum of the model rat during different modelling time points.Results:Compared with the control group

the weight of the model rats has no significant change different from that of weight-increasing control group from the 1～3 week.The weight is lighter still though it brings back a little in the 4 th week(P<0.001);The weight of thymus and spleen of the model rats is lighter significantly than that of the control group(P<0.05 or P<0.01 or P<0.001).The index of thymus decreased significantly in the 1 st week(P<0.01) but came back in the 3 rd、4 th week.The index of spleen increased significantly compared with that of the control group in the 3 rd and 4 th week(P<0.05).The interleukin-1β(IL-1β) in serum of the model group increased obviously in the 1 st week(P<0.05)

and decreased significantly in the 4 th week(P<0.01).The interleukin-2(IL-2) in serum of the model group decreased in the 3 rd week and trended to increase again in the 4 th week.The interleukin-6(IL-6) decreased gradually during the 4 weeks.The tumor necrosis factor α(TNF α) trended to increase from 1～3 week

and trended to degrade in the 4 th week.Conclusion:After three weeks

the weight of thymus and the weight of spleen are reduced.In the 4 w the weight of thymus and spleen has no recovery.During the course serum IL-1β degraded continuously

and IL-2、IL-6、TNFα changed disorderly.So it hints the syndrome of spleen-qi deficiency brings about the changes in immunological system.