HPLC Fingerprint Analysis of (Thunb) Vahl in Different Collecting Seasons
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HPLC Fingerprint Analysis of (Thunb) Vahl in Different Collecting Seasons
Chinese Journal of Experimental Traditional Medical FormulaeVol. 13, Issue 3, Pages: 1-4(2007)
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Published:2007
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ZHENG Xiao-ke, WEI Yue, FENG Wei-sheng. HPLC Fingerprint Analysis of (Thunb) Vahl in Different Collecting Seasons[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(3): 1-4.
DOI:
ZHENG Xiao-ke, WEI Yue, FENG Wei-sheng. HPLC Fingerprint Analysis of (Thunb) Vahl in Different Collecting Seasons[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(3): 1-4.DOI:
HPLC Fingerprint Analysis of (Thunb) Vahl in Different Collecting Seasons
Objective:To compare HPLC fingerprint of Forsythia Suspense(Thunb)Vahl in different collecting seasons for determining the optimum harvesting time.Methods: The experimental condition of the RP-HPLC method was as follows: Shim-pack CLC-ODS column(150×4.6 mm)
with linear gradient elution using acetonitrile and water; The flow rate was 0.8 mL/min;Detected wavelength was 235 nm;The column temperature was 25 ℃.The HPLC fingerprints were analyzed by similarity evaluation and principle component analysis to conduct the chemical pattern recognition.Results: The content of chemical composition of Forsythia Suspense(Thunb) Vahl varied according to collecting seasons.The accumulation of constituents showed a regular pattern.Conclusion:The study offers the basis of controlling the quality of Forsythia Suspense(Thunb) Vahl and the realization of good agricultural practice.