Objective:To explore the impact of Xuefuzhuyv Decoction on serum proteome of rat with syndrome of Qi-stagnancy and blood stasis and to investigate the molecular mechanism of Xuefuzhuyv Decoction.Methods:The rat models with syndrome of Qi-stagnancy and blood stasis were established by combined methods.The rat serum was collected from normal group

model group and treated group.A simple method was employed to remove the high abundance albumin in serum.Proteomic protocol included proteome separation with two dimensional gel electrophoresis (2-DE)

gel image analysis with Image Master 2D Elite 6.01 system

and identification of the different proteins between the three groups.Results:(354±20) proteins had been observed in normal group serum

(347±37) in model group serum

and(360±35) in treated group serum.Compared with normal group

seven changed proteins were determined: one protein decreased

five proteins increased and one protein lost in rat serum of model group

but these changes could be improved by treating Xuefuzhuyv Decoction.Conclusions:The molecular mechanism of Xuefuzhuyv Decoction which could treat the rat with syndrome of Qi-stagnancy and blood stasis may associated with those different proteins

and it will provide a new idea for study of molecular mechanism of Xuefuzhuyv Decoction.