Preliminary Studies on Standardization of Cerebrospinal Fluid Pharmacological Experimental Methods——Protective Effect of CSF of Optimized Danggui Shaoyao San on PC12 Cells

ZHANG Qi-chun1; KOU Jun-ping2; ZHU Dan-ni2; WANG Qiu-juan1; YAN Yong-qing2

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Preliminary Studies on Standardization of Cerebrospinal Fluid Pharmacological Experimental Methods——Protective Effect of CSF of Optimized Danggui Shaoyao San on PC12 Cells

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- Preliminary Studies on Standardization of Cerebrospinal Fluid Pharmacological Experimental Methods——Protective Effect of CSF of Optimized Danggui Shaoyao San on PC12 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 13, Issue 8, Pages: 10-13(2007)
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- Published：2007
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ZHANG Qi-chun1, KOU Jun-ping2, ZHU Dan-ni2, et al. Preliminary Studies on Standardization of Cerebrospinal Fluid Pharmacological Experimental Methods——Protective Effect of CSF of Optimized Danggui Shaoyao San on PC12 Cells[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(8): 10-13.
DOI：

ZHANG Qi-chun1, KOU Jun-ping2, ZHU Dan-ni2, et al. Preliminary Studies on Standardization of Cerebrospinal Fluid Pharmacological Experimental Methods——Protective Effect of CSF of Optimized Danggui Shaoyao San on PC12 Cells[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(8): 10-13. DOI：

摘要

目的:通过观察当归芍药散精简方(FBD)含药脑脊液对过氧化氢所致PC12细胞损伤模型的保护作用

对脑脊液药理实验条件规范化进行初步探讨。方法:以MTT法观察不同实验条件下(给药方案、添加量、处理方法、批次及保存时间)

FBD大鼠含药脑脊液对过氧化氢损伤的PC12细胞存活率的影响。结果:过氧化氢损伤的PC12细胞存活率随FBD含药脑脊液添加量增加而提高;相同添加量多次给药(2次/d×3.5d)含药脑脊液的保护率均高于单次给药的含药脑脊液;未处理含药脑脊液与经56℃、乙醇、丙酮处理含药脑脊液对PC12细胞均有显著的保护作用(P<0.01)

各组保护率无明显差异;同时观察到不同批次大鼠及不同保存时间的含药脑脊液对受损PC12细胞均有显著保护作用

但随保存时间的延长

其保护率下降。结论:含药脑脊液的制备可采用连续多次给药方案

无需灭活处理

一般以体外添加终体积的10%

-20℃冻存不超过30d为宜。

Abstract

Objective:To explore the standard conditions of the cerebrospinal fluid pharmacological method

by investigating the neural protective effect of cerebrospinal fluid(CSF) containing FBD.Methods:The effect of rat CSF with FBD on injured PC12 cell induced by hydrogen peroxide was observed by MTT assay under various experimental conditions(dosage regiment

addition level

treatment process).Results:The viability of PC12 cell injured by hydrogen peroxide increased with the addition level of CSF with FBD

and at the same addition level(5%

10%

20%)

CSF of rat after administrating FBD repetitiously (2 times/d)×3.5 d) exerted higher protective efficacy on PC12 cell than that of after administrating FBD once.The raw CSF with FBD and the CSF with FBD treated by heat(56 ℃)

ethanol

acetone all had notable protective effects on PC12 cell(P<0.01)

and there was no significant statistical difference among them.Meanwhile

CSF with FBD of different batch of rats and with different preserved time also showed distinct activities

however

protective rate declined with prolonging of the preserved time.Conclusion:It is proposed that the CSF containing drugs may be prepared with 10% rat CSF without treating after administrating drugs repetitiously and preserved at -20 ℃ no more than 30 days.

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Preliminary Studies on Standardization of Cerebrospinal Fluid Pharmacological Experimental Methods——Protective Effect of CSF of Optimized Danggui Shaoyao San on PC12 Cells

Related Author

ZHANG Qi-chun~1

KOU Jun-ping~2

ZHU Dan-ni~2

WANG Qiu-juan~1

YAN Yong-qing~

Related Institution

中国药科大学中药复方研究室

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