Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC

WANG Xu1; JIN Xiang-qun1; SUI Cheng2

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Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC

更新时间：2024-01-04

- Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 3, Pages: 22-23(2008)
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- Published：2008
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WANG Xu1, JIN Xiang-qun1, SUI Cheng2. Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(3): 22-23.
DOI：

WANG Xu1, JIN Xiang-qun1, SUI Cheng2. Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(3): 22-23. DOI：

摘要

目的:建立高效液相色谱法测定双贯感宁颗粒中绿原酸含量的方法。方法:采用十八烷基键合硅胶色谱柱(250mm×4.6mm

5μm)

以乙腈-0.4%磷酸溶液(13∶87)为流动相

流速为1.00mL·min-1

检测波长为327nm。结果:绿原酸的线性范围为(0.04～0.37)μg(r=0.9998)

平均回收率(n=5)为99.50%(RSD=0.84%)。结论:本方法简单、迅速、可靠。

Abstract

Objective:To develop an HPLC method for determining chlorogenic acid in Shuangguan Ganning Granule.Methods: Diamonsil C18 column(250 mm×4.6 mm

5 μm)was used as the stationary phase

the mixture of acetonitrile-0.4% phosphoric acid(13∶87) was served as mobile phase

and its flow rate was 1.0 mL·min-1.The wavelength for detection was 327 nm.Results:The linearity range was(0.04～0.37) μg(r=(0.999 8).) The average recovery was 99.50%(RSD=0.84%).Conclusion: The method is simple

fast and reliable.

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Related Institution

College of Traditional Chinese Medicine,Capital Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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