In Vitro Studies of Gubenyiliu Prescription Ⅱ and Its Functional Compositions on A Lung Carcinoma Cell and Synergistic Action with Chemotherapy

ZHANG Gan-lin1; 2; LI Ping1; WANG Xiao-min3; TANG Yong3; LIU Xin1; SHENG Xun1

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In Vitro Studies of Gubenyiliu Prescription Ⅱ and Its Functional Compositions on A Lung Carcinoma Cell and Synergistic Action with Chemotherapy

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- In Vitro Studies of Gubenyiliu Prescription Ⅱ and Its Functional Compositions on A Lung Carcinoma Cell and Synergistic Action with Chemotherapy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 13, Issue 1, Pages: 35-38(2007)
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- Published：2007
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ZHANG Gan-lin1, 2, LI Ping1, et al. In Vitro Studies of Gubenyiliu Prescription Ⅱ and Its Functional Compositions on A Lung Carcinoma Cell and Synergistic Action with Chemotherapy[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(1): 35-38.
DOI：

ZHANG Gan-lin1, 2, LI Ping1, et al. In Vitro Studies of Gubenyiliu Prescription Ⅱ and Its Functional Compositions on A Lung Carcinoma Cell and Synergistic Action with Chemotherapy[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(1): 35-38. DOI：

摘要

目的:探讨益气活血中药固本抑瘤Ⅱ号(全方)和各拆方组药物对A549肺癌细胞生长的影响及与化疗药物顺铂(DDP)联合应用的效果。方法:固本抑瘤Ⅱ号全方和根据功效拆分的益气组、行气活血组、培补肝肾组、散结消组药物

分别制备水提物。采用MTT法检测各组药物对A549细胞生长的影响及与DDP联合作用的效果。结果:单独应用全方和行气活血组水提物对A549细胞生长表现为抑制作用

IC50分别为33.21 mg生药/mL和9.55 mg生药/mL;与DDP合用表现为增效作用

增效倍数分别为1.23和1.77

合并指数为1.05和0.83

表现出协同和相加作用。结论:固本抑瘤Ⅱ号在体外实验中能够抑制A549细胞生长

与DDP合用有一定的化疗增效作用;行气活血组药物也具有抑瘤和增效作用

且作用与全方接近。提示行气活血组中药为体外实验中全方抗肿瘤作用及化疗增效作用的有效组分。

Abstract

Objective:To study the effect of Gubenyiliu Prescription Ⅱ(Prescription Ⅱ

PⅡ) and its functional groups on A549 lung carcinoma cell's growth and combinative effect with diamminedichloroplatinum(DDP).Methods:According to its functional composition

Prescription Ⅱ was divided into four groups:Reforcing vital energy group(VE)、Promoting qi to activate blood group(QB)、Cultivating kidney and liver group(KL)and Eliminating stagnation and removing lump group(SL).Their aqueous extracts were prepared respectively.MTT assay was used for A549 growth and combinative effect with DDP.Results:Both Prescription Ⅱ and QB group showed depressive effect on A549 growth.The IC50 was 33.21 mg crude drug/mL and 9.55 mg crude drug/mL respectively.Both of them had synergistic action with DDP

the multiple was 1.23 and 1.77

complicating index was 1.05 and 0.83 dccordingly.Conclusion:PⅡ can inhibit A549 grow

and it has synergistic action with DDP to some extent.Among the functional groups

QB group has the similar effects with PⅡ.It indicates that herbs in QB group play the main active effect against tumour and synergetic action in the whole prescription.

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In Vitro Studies of Gubenyiliu Prescription Ⅱ and Its Functional Compositions on A₅₄₉ Lung Carcinoma Cell and Synergistic Action with Chemotherapy

Related Author

ZHANG Gan-lin~

李萍

王笑民

唐勇

刘欣

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Related Institution

1,2),LI Ping~1,WANG Xiao-min~

首都医科大学附属北京中医医院肿瘤科

北京市中医研究所北京100010

北京中医药大学中药学教研室

北京100029

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