Objective:To develop an HPLC method to determine evodiamine

rutaecarpine and evodin simultaneously in cut crude drug and exact of fructus evodiae.Methods:The separation was performed on the Kromasail C18(4.6 mm×250 mm

5 μm) column with acetonitrile-water-tetrahydorfuran-acetic acid(51∶48∶1∶0.1) as mobile phase.The flow rate was 1 mL·min-1

the wavelength of UV detector was set at 225 nm and the column temperature was set at 32 ℃.Results:The linear range of evodiamine

rutaecarpine and evodin were 0.015～0.3 μg(r=(0.999 9))

0.012～0.24 μg(r=(0.999 9))

0.059～1.18 μg(r=(0.999 6)) respectively.The average recovery of evodiamine was 99.6%(n=5)

RSD0.65%;The average recovery of rutaecarpine was 99.1%(n=5)

RSD1.2%;The average recovery of evodin was 97.5%(n=5)

RSD 1.4% respectively.Conclusion:This method was simple

rapid and accurate.