Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC

WANG Guo-hua; LI Hui; NIE Qi-xia; ZANG chen; ZHANG Bao-xian

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Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC

更新时间：2024-01-04

- Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 13, Issue 6, Pages: 14-16(2007)
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- Published：2007
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WANG Guo-hua, LI Hui, NIE Qi-xia, et al. Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(6): 14-16.
DOI：

WANG Guo-hua, LI Hui, NIE Qi-xia, et al. Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(6): 14-16. DOI：

摘要

目的:建立高效液相色谱法测定退障凝胶中大黄素、大黄酚含量的方法。方法:采用Waters高效液相色谱仪(Waters 2695-2487系统);Empower色谱工作站;色谱柱Kromasil C18(4.6mm×250mm

5μm);流动相为甲醇-0.1%磷酸溶液(82∶18);紫外检测波长254nm;流速1.0mL·min-1;进样量20μL。结果:大黄素在0.02～0.125μg范围内呈现良好的线性关系

大黄酚在0.072～0.360μg范围内呈现良好的线性关系

大黄素、大黄酚的加样回收率分别为99.1%

99.2%。结论:该法简便

准确

具有专属性

可用于退障凝胶的质量控制。

Abstract

Objective:To develop an HPLC method for analysis of emodin and chrysophanol in Tuizhang Hydrogel.Method:The emodin and chrysophanol in Tuizhang Hydrogel were separated on a Chromasil C18 column(4.6 mm×250 mm

5 μm) eluted with the mobile phase of methanol-0.1% Phosphoric acid(82∶18).The flowrate was 1.0 mL·min-1

and the UV detector wavelenghth was set at 254 nm.Results:The calibration curves were linear over the concentration range of 0.025～0.125 μg for emodin and 0.072～0.360 μg for chrysophanol.The recoveries of the emodin and chrysophanol were 99.1%

99.2%

respectively.Conclusion:The method was simple and accurate

it can be used for the determination of emodin and chrysophanol in Tuizhang Hydrogel.

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Related Institution

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School of Pharmacy,Anhui Medical University

Department of Pharmacy,Third-Grade pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine,The First Affiliated Hospital of Anhui Medical University

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