Determination of Icariin in Ronggu Granule by HPLC

ZHANG Xiu-mei1; WANG Jin1; HE Wei2

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Determination of Icariin in Ronggu Granule by HPLC

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- Determination of Icariin in Ronggu Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 13, Issue 2, Pages: 10-11(2007)
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- Published：2007
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ZHANG Xiu-mei1, WANG Jin1, HE Wei2. Determination of Icariin in Ronggu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(2): 10-11.
DOI：

ZHANG Xiu-mei1, WANG Jin1, HE Wei2. Determination of Icariin in Ronggu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(2): 10-11. DOI：

摘要

目的:应用高效液相色谱(HPLC)法测定荣骨颗粒中淫羊藿苷的含量

控制该制剂的质量。方法:采用Zorbax-SB-C18柱(5 μm

4.6×150 mm)分离淫羊藿苷

以甲醇-水(60∶40)为流动相

检测波长为270 nm。结果:淫羊藿苷线性范围为0.1～1.0 μg

r>=0.999 9

平均回收率为101.7%

RSD=1.86%(n=5)。结论:该方法简便、可靠、准确

可用于该制剂的质量控制。

Abstract

Objective:To determine the content of Icariin in Ronggu Granule by HPLC for quality control.Methods:Zorbax-SB-C18(5 μm

4.6×150 mm) column was used with methanol∶water(60∶40)as mobile phase

and the determination wavelength was set at 270 nm.Results:The linear range was 0.1～1.0 μg for Icariin(r=(0.999 9))

the average recovery was 101.7% and RSD was 1.86%(n=5).Conclusion:This method is simple

reliable and accurate for the determination of icariin

it can be used for quality control of this preparation.

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Related Institution

The First Clinical Medical College of Guangzhou University of Chinese Medicine

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences

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Level 3 Laboratory for State Administration of Traditional Chinese Medicine, Beijing Zhendong Gguangming Pharmaceutical Research Institute Co.Ltd.

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