Protective Effects of the Serum containing Tianma Gouteng Yin on Human Umbilical Vein Endothelin Cells
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Protective Effects of the Serum containing Tianma Gouteng Yin on Human Umbilical Vein Endothelin Cells
Chinese Journal of Experimental Traditional Medical FormulaeVol. 13, Issue 1, Pages: 26-28(2007)
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Published:2007
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MENG Yun-hui1, TU Xin2, TU Jin-wen3, et al. Protective Effects of the Serum containing Tianma Gouteng Yin on Human Umbilical Vein Endothelin Cells[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(1): 26-28.
DOI:
MENG Yun-hui1, TU Xin2, TU Jin-wen3, et al. Protective Effects of the Serum containing Tianma Gouteng Yin on Human Umbilical Vein Endothelin Cells[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(1): 26-28.DOI:
Protective Effects of the Serum containing Tianma Gouteng Yin on Human Umbilical Vein Endothelin Cells
Objective:To observe protective effects of Tianma Gouteng Yin on the injury of human umbilical vein endothelin cells(HUVECs)induced by angiotensin Ⅱ(AngⅡ).Methods:The serum containing metabolic ingredients of Tianma Gouteng Yin was taken from rats.HUVECs were cultured in vitro by collagenase digestive method and identified with human von Willebrand factor immunocytochemical method.HUVECs were randomly divided into three groups:control group
AngⅡ group
AngⅡ and Tianma Gouteng Yin group.The morphology and density of HUVECs were observed with invert microscope.The content of TNF-α in supernatant medium was detected with enzyme-linked immunoadsorbent assay.The expression of peroxisome proliferator-activated receptor γ was measured by means of reverse transcription polymerase chain reaction.Results:Compared with control group
AngⅡ(10-6mol/L) could make the density of HUVECs to decrease and the secretion of TNF-α to increase.The expression of peroxisome proliferator-activated receptor γ was lowed.The serum containing metabolic ingredients of Tianma Gouteng Yin could inhibit the decrease of HUVECs and decrease the secretion of TNF-α and increasethe expression of peroxisome proliferator-activated receptor γ.Conclusion:Tianma Gouteng Yin could inhibit the injury of HUVECs induced by AngⅡ.