Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner

ZHOU Xuan; SONG Fen-Yun; ZHONG Zhao-jian

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Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner

更新时间：2024-01-04

- Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 12, Issue 8, Pages: 14-15(2006)
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- Published：2006
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ZHOU Xuan, SONG Fen-Yun, ZHONG Zhao-jian. Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 14-15.
DOI：

ZHOU Xuan, SONG Fen-Yun, ZHONG Zhao-jian. Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 14-15. DOI：

摘要

目的:测定槐米中芦丁和槲皮素的含量。方法:以微乳液十二烷基硫酸钠-正丁醇-正己烷-水-甲酸为展开剂

在聚酰胺薄膜上成功地分离了槐米中的芦丁和槲皮素

并以370nm为测定波长

550nm为参比波长对之进行扫描测定。结果:芦丁和槲皮素的线性范围分别为0.2～3.0μg、0.1～1.0μg;回收率分别为98.8%和97.1%。结论:建立了一种新的同时测定槐米中芦丁和槲皮素含量的薄层色谱扫描法。

Abstract

Objective:To develop a TLCS method for determination of rutin and quercetin in Flos sophorae.Method:A TLCS method was used.The developer was a micro-emulsion consisted of soldium dodecyl sulphate/n-C4H9OH/n-C6H14/H2O/formicacid(0.27∶0.63∶0.10∶3∶0.2) and the thin layer was polyamide film.The spots of rutin and quercetin were well separated and scanned at 370nm with reference wavelength of 550nm.Results:The linear ranges of calibration curves were 0.2～3.0μg for rutin

0.1～1.0μg for quercetin.The recoveries of rutin and quercetin were 98.8% and 97.1% respectively.Conclusion:A TLCS method has been developed for the simultaneous determination of rutin and quercetin in Flos sophorae.

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Related Institution

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