Determination of Quercetin in Hawthorn Leaves by HPLC

WEI Hua-ling; YANG Qing; WEI Hong-yin

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Determination of Quercetin in Hawthorn Leaves by HPLC

更新时间：2024-01-04

- Determination of Quercetin in Hawthorn Leaves by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 12, Issue 8, Pages: 10-11(2006)
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- Published：2006
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WEI Hua-ling, YANG Qing, WEI Hong-yin. Determination of Quercetin in Hawthorn Leaves by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 10-11.
DOI：

WEI Hua-ling, YANG Qing, WEI Hong-yin. Determination of Quercetin in Hawthorn Leaves by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 10-11. DOI：

摘要

目的:用高效液相色谱法(HPLC法)测定山楂叶中槲皮素的含量。方法:采用C18柱

以甲醇:0.4%磷酸(45∶55)为流动相

流速为0.9mL/min

检测波长为360nm。结果:槲皮素在0.1624～1.624μg/mL范围内与峰面积线性关系良好

Abstract

Objective:To develop a HPLC method for determining the content of quercetin in hawthorn leaves.Methods:The Suntek ODS column with methanol-0.4% phosphoric acid(45∶55) as the mobile phase was used.The flow rate was 0.9mL/min and the detective wavelength was 360nm.Results:The linear range of calibration curve was 0.1624～1.624μg/mL

for the quercetin(r=0.9998).The average recovery was 97.9% and the RSD was 0.59%(n=6).Conclusion:The method is simple

and accurate with a good repeatability and can be used as quantitative analysis for this preparation.

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