Objective:To study the influence of Shengmai Injection(SMI) on the tissue concentration of digoxin(DG) in rats with heart failure(HF).Methods:40 model rats with HF were randomly divided into 4 groups:the control group(treated with DG only) and other 3 groups(treated with DG plus SMI 4mL/kg

8mL/kg

12mL/kg respectively).The DG concentrations in the myocardium

liver

kidney and skeletal muscle were determined by radio immunoassay(RIA) at the designed time after administration.Result:There was no significant difference in the DG concentrations of myocardium and liver among 4 groups after administration.At the 30min and 12h after administration

the DG concentrations of kidney for the SMI 12mL/kg group were significantly higher than the other 3 ones respectively(P<0.05).The DG concentrations of the skeletal muscle in the SMI 8mL/kg and 12mL/kg groups at 30min and the SMI 12mL/kg at 12h were significantly higher than the control and SMI 20mL groups(P<0.05 or P<0.01).Conclusion:There was the tendency of the increased DG concentrations of kidney in the SMI 12mL/kg group and of skeletal muscle in the SMI 8mL/kg and 12mL/kg groups.