Mechanisms of Qing Yi decoction on Acute Lung Injury Induced by Acute Hemorrhagic Necrotizing Pancreatitis in Rats

LI Dong-hua; LIU Hong-bin; CUI Nai-qiang; WANG Qian; XUE Xiao-ping

您当前的位置：

首页 >

文章列表页 >

Mechanisms of Qing Yi decoction on Acute Lung Injury Induced by Acute Hemorrhagic Necrotizing Pancreatitis in Rats

更新时间：2024-01-04

- Mechanisms of Qing Yi decoction on Acute Lung Injury Induced by Acute Hemorrhagic Necrotizing Pancreatitis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 12, Issue 5, Pages: 43-46(2006)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2006
- 稿件说明：
移动端阅览
LI Dong-hua, LIU Hong-bin, CUI Nai-qiang, et al. Mechanisms of Qing Yi decoction on Acute Lung Injury Induced by Acute Hemorrhagic Necrotizing Pancreatitis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(5): 43-46.
DOI：

LI Dong-hua, LIU Hong-bin, CUI Nai-qiang, et al. Mechanisms of Qing Yi decoction on Acute Lung Injury Induced by Acute Hemorrhagic Necrotizing Pancreatitis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(5): 43-46. DOI：

摘要

目的:研究清胰汤(QYT)对大鼠急性出血坏死性胰腺炎(AHNP)肺损伤的治疗机理。方法:Wistar大鼠经胆胰管逆行注射5%牛磺胆酸钠(1mL/kg

0.1mL/min)诱发AHNP;QYT组在造模后30min灌胃QYT(10mL/kg);善宁组在造模后30min皮下注射善宁(50μg/kg)。每组又分别随机分为36、12h三组(每组8只)

其余12只用于观察24小时死亡率。于不同时间点测定血清淀粉酶(AMY)、脂肪酶(LIP)、磷脂酶A 2(PLA2)、肿瘤坏死因子(TNFα);测定肺组织髓过氧化物酶(MPO)、PLA2、TNFα;提取肺泡灌洗液巨噬细胞核蛋白

ELISA法检测核因子(NF-κBP65)表达;对肺、胰腺组织行病理检查。结果:QYT明显降低AHNP大鼠死亡率;减轻肺、胰腺病理损伤;降低血清、肺组织PLA2、TNFα水平;显著降低肺泡巨噬细胞NF-κB活性。结论:清胰汤可通过其多途径多靶点作用

抑制PLA2活性

抑制肺内炎症相关细胞NF-κB活化

下调多种细胞因子表达

减轻肺损害

降低死亡率。

Abstract

Objective:To investigate the therapeutic mechanisms of Qing Yi decoction on acute lung injury induced by acute hemorrhagic necrotizing pancreatitis in rats.Methods:AHNP model was induced by intraductul administration of 5% sodium taurocholate in Wistar rat.Qing Yi decoction was administered orally 30 minutes after model induction.Sandostain(50μg/kg) was administrated by subcutaneous injection after model induction.The other animals were used to observe the 24hour-mortality.Animals were killed on various hours after model induction(3

12h).Bronchoalveolar lavage(BAL) macrophages NF-κ B activation was determined by ELISA method.Pulmonary TNFα

PLA2

myeloperoxidase(MPO)were examined.The Levels of Amylase

Lipase

PLA2 of serum were determined also.Morphologic changes of lung and pancreas were observed under light microscopy.Results:In Qing Yi decoction group

the mortality was significantly reduced;Lung and pancreas injury was markedly reduced;Qing Yi decoction significantly attenuated NF-κ B activation of BAL macrophages.PLA2

TNFα levels of serum and lung tissue were decreased significantly.Conclusions:Qing Yi decoction has protective effects on AHNP-ALI through multiple pathways

among these

the inhibitory effect on PLA2 activity is an important one.Qing Yi decoction can attenuate the "PLA2-NF-κ B-cytokine cascade" markedly.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Jiebiao Qingli Decoction Regulates TLR7/MAPK/NF-κB Pathway to Prevent and Treat Pneumonia Induced by IAV Infection

Role of Cytokines and Related Signaling Pathways in Intervention of Chinese Medicine in Radiation-induced Lung Injury： A Review

Shaoyaotang and Its Modified Formulas in Treatment of Ulcerative Colitis： A Review

Immunoregulatory Effect of Jiedu Tongluo Shengjin Prescription on Sjögren's Syndrome Mice Based on Stability of Treg Cells

Molecular Mechanism of "Transmission Between Lung and Brain" of Influenza and Intervention Effect of Maxing Shigantang Based on JAK1/STAT1 Signaling Pathway

Related Author

MING Yu

MA Yichuan

YAO Ruiqi

CHAO Yan

ZHANG Hongchun

SHAO Jiayi

WU Mianhua

MA Yanxia

Related Institution

Clinical Research Center for Respiratory Diseases，China-Japan Friendship Hospital

Beijing Oriental Yunjia Pharmaceuticals Co. Ltd.

Shenzhen Nanshan District Hospital of Traditional Chinese Medicine

Shenzhen Bao'an Authentic Traditional Chinese Medicine Therapy Hospital

Institute of Basic Research in Clinical Medicine，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰