Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin

GUI Shuang-ying; ZHOU Ya-qiu; KE Zhong-cheng

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Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin

更新时间：2024-01-04

- Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 12, Issue 2, Pages: 25-27(2006)
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- Published：2006
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GUI Shuang-ying, ZHOU Ya-qiu, KE Zhong-cheng. Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 25-27.
DOI：

GUI Shuang-ying, ZHOU Ya-qiu, KE Zhong-cheng. Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 25-27. DOI：

摘要

目的:研究大孔吸附树脂分离富集赤芍中芍药苷的性能。方法:采用HPLC法测定芍药苷的含量;考察大孔吸附树脂对芍药苷的吸附性能以及洗脱参数。结果:D101大孔吸附树脂对提取液中芍药苷的最佳分离条件为:上柱液浓度为2.5mg/mL、流速为3.6BV/h

芍药苷比吸附量达32.2mg/g

20%乙醇洗脱率为91.25%

芍药苷纯度可达73.70%。结论:D101大孔吸附树脂可用于赤芍中芍药苷的分离富集。

Abstract

Objective:To study the separation effect of D101 macroporous resin for the purification of Paeoniflorin in Radix Paeonia.Methods:HPLC was used to analyze the content of Paeoniflorin

and the adsorption performance and the elution parameters were investigated.Results:The optimal separation conditions were as follows:the concentration of Paeoniflorin was 2.5mg/mL with a flow rate of 3.6BV/h

and 20% alcohol was used as eluant.The adsorption of Paeoniflorin was 32.2mg/g

and the elution ratio of Paeoniflorin was 91.25%

and the purity of Paeoniflorin reached 73.70%.Conclusion:D101 resin can be used to refine the Paeoniflorin in the extraction of Radix Paeonia.

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