Protective Effects of Total Flavone from the Flowers of Ablemoschl manihot L. Medic on the Myocardial Ischemia/Reperfusion Injury
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Protective Effects of Total Flavone from the Flowers of Ablemoschl manihot L. Medic on the Myocardial Ischemia/Reperfusion Injury
Chinese Journal of Experimental Traditional Medical FormulaeVol. 12, Issue 2, Pages: 39-42(2006)
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Published:2006
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LI Qing-lin1, WANG Cheng-yong1, PENG Dai-yin1, et al. Protective Effects of Total Flavone from the Flowers of Ablemoschl manihot L. Medic on the Myocardial Ischemia/Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 39-42.
DOI:
LI Qing-lin1, WANG Cheng-yong1, PENG Dai-yin1, et al. Protective Effects of Total Flavone from the Flowers of Ablemoschl manihot L. Medic on the Myocardial Ischemia/Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 39-42.DOI:
Protective Effects of Total Flavone from the Flowers of Ablemoschl manihot L. Medic on the Myocardial Ischemia/Reperfusion Injury
Object:To study the effects and mechanism of total flavone from the flowers of Ablemoschl Manihot L.Medic(Tfa) against myocardium injury induced by ischemia and reperfusion.Methods:The ischemia-reperfusion model was induced by after 30 min of coronary occlusion followed by 3.5 h of reperfusion in rats.The creatine phosphokinase(CPK) of serum
the malondialdehyde(MDA) and the superoxide dismutase(SOD) of myocardium were measured.The cell injure model in the cultured neonatal rat cardiomyocytes was induced by anoxia-reoxygenation and remove oxygen and glucose from medium
and the lactate dehydrogenase(LDH) was detected.Apoptosis was determined by terminal deoxy-nucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling(TUNEL) method and Bcl-2 expression determined by immunohistochemistry method.Results:The results showed that Tfa can decreased the MDA contents in myocardium and the production of CPK in serum
improved the SOD activitie;also decreased the number of apoptotic cells and enhanced the Bcl-2 expression in injured rat myocardium by ischemia/reperfusion.Tfa showed it can reduced the release of LDH from injured cardiomyocytes.Conclusion:Tfa showed the protective effect against myocardial ischemia and reperfusion injured which might be resist the damaging of oxygen free radical(OFR) and inhibited cardiomyocyte apoptosis.
Mechanism of Ethoxysanguinarine in Regulating IRE1/RIDD Signaling Pathway to Inhibit Endoplasmic Reticulum Stress and Alleviate Cardiomyocyte Apoptosis
Advancement of Apoptosis
Influence of Breviscapine on Ultrastructure and Apoptosis of Myocardial Cellsafter Myocardial Ischemia and Eperfusion in Rat
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