Ligustrazine-induced Alterations in Gene Expression in Human Umbilical Vein Endothelial Cells
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Ligustrazine-induced Alterations in Gene Expression in Human Umbilical Vein Endothelial Cells
Chinese Journal of Experimental Traditional Medical FormulaeVol. 11, Issue 3, Pages: 40-43(2005)
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Published:2005
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WU Hong-jin1, LU Jun-ping1, MA Zeng-chun2, et al. Ligustrazine-induced Alterations in Gene Expression in Human Umbilical Vein Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2005, 11(3): 40-43.
DOI:
WU Hong-jin1, LU Jun-ping1, MA Zeng-chun2, et al. Ligustrazine-induced Alterations in Gene Expression in Human Umbilical Vein Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2005, 11(3): 40-43.DOI:
Ligustrazine-induced Alterations in Gene Expression in Human Umbilical Vein Endothelial Cells
To investigate the effect of Ligustrazine on the spectrum of gene expression in the cultrured human umbilical vein endothelial cells(HUVECs)
and further explore the potential molecule mechanism of endothelial protection by Ligustrazine on HUVECs.Method:A home-made oligonucleotide microarray containing 500 cardiovascular disease-related genes was constructed.The alteration of the spectrum of gene expression induced by Ligustrazine in HUVECs was detected by oligonucleotide microarray analysis.Results:7 up-regulated and 18 down-regulated genes were obtained after Ligustrazine (40ug/mL) had acted on HUVECs for 24hours.Ligustrazine affected the expression levels of genes involved in immunization
vasomotion
cell adherence
coagulation
antioxidation
cell growth
signal transduction and substance metabolism in HUVECs.Conclusions:Ligustrazine regulated sets of genes in endothelial cells and protected the cells.Microarray analysis provided us valuable insights into the protective mechanism by Ligustrazine.
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