Objects: To investgigate the influence of Qingzhi capsule on platelet intraceller cAMP and cGMP levels

intraceller calcium of platelet ([Ca2+]i)

plasma PGE2

TXB2and 6-Keto-PGF1α in two models of LPS attack in rats.Methods:Two kinds of model were established: after oral administration of Qingzhi capsule 8d

attack was induced by intraperitoneal injecting(i.p.)LPS; another model was established with high fat diet for 8d

then induced by i.p.LPS at the last day.At 6h or 24h of post-supplementation rats were anesthetized

blood was collected immediately from abdominal aorta.The levels of Plasma TXB2and 6-Keto-PGF1αwas assayed by radioimmunoassay (RIA)

cAMP and cGMP levels of platelet were assayed by RIA too.[Ca2+]i were examined with flow cytometer.The level of Plasma PGE2was measured by enzyme-immunoassay(EIA).Results and Conclusion:Qingzhi Capsule increased the level of platelet [Ca2+]i on 24h after LPS attack

Qingzhi Capsule improved the levels of Plasma PGE2and 6-Keto-PGF1αat 24h in the rats with only LPS attack.Qingzhi Capsule had strong effect on prevention of increasing TXB2.Results also indicated depressing of TXB2/6-Keto-PGF1α ratio in the rats with high fat diet and LPS attack.