Studies on Purification of Saponins from Radix Polygalae with Macroporous Resin
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Studies on Purification of Saponins from Radix Polygalae with Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 10, Issue 6, Pages: 3-6(2004)
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Published:2004
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LIU You-ping, WAN De-Guang, YAN dan, et al. Studies on Purification of Saponins from Radix Polygalae with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(6): 3-6.
DOI:
LIU You-ping, WAN De-Guang, YAN dan, et al. Studies on Purification of Saponins from Radix Polygalae with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(6): 3-6.DOI:
Studies on Purification of Saponins from Radix Polygalae with Macroporous Resin
Objectives: To study the technological parameters of purifying saponin with macroporous resin. Method: With the elution rate and purity as indexes
the absorption characteristics and elution parameters were studied. Result: 12 mL of the extractive of Radix Polygalae (crude drugs 0.25g/mL) was added on a column of macroporous resin (Φ15mm×H 90mm
dry weight 2.5g) and was washed with 3BV of distilled water
30% ethanol and 70% ethanol
successively. Saponin was enriched in the 70% ethanol elution. Conclusion:Enriched and purified by macroporous resin
the elution rate of saponin was 89.8% and the purity reached 239.6%. So