Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice
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Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice
Chinese Journal of Experimental Traditional Medical FormulaeVol. 10, Issue 2, Pages: 18-21(2004)
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Published:2004
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Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(2): 18-21.
DOI:
Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(2): 18-21.DOI:
Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice
Objective:To distinguish the active fraction extracted from the optimized Danggui Shaoyao San(FBD)
which can prevent and treat vascular dementia.Method:(1) The dysmnesia model of learning and memory induced by scopolamine and ischemia-reperfusion was used. The ability of learning and memory was measured by step-through task and water maze task. (2) PC12 cell was injured by N`a2S2O4 and glutamine and the viability of cell was measured by MTT method.Results:At the same dosage of herbal medicine
the three compositions composed by four fractions extracted from FBD
[Polysaccharide+Volatile oil]
[Polysaccharide+Volatile oil+n-Butanol-fraction] and [Polysaccharide+Volatile oil +Water-fraction]
shortened swimming time obviously (P<0.05)
prolonged significantly step-through latency and decreased number of errors in 5 minutes (P<0.05~0.01). (2) 10% Serum containing FBD and the three compositions were manifested to increase viability of injured PC12 cell (P<0.1~0.05). [Polysaccharide+Volatile oil] worked most obviously (P<0.05~0.01) at behavior and cellular levels.Conclusion: [Polysaccharide+Volatile oil] might be the active fraction of optimized Danggui Shaoyao San for preventing and treating VD.
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Related Author
FENG Xiaoxia
CHEN Chen
QIU Jing
YANG Guang
DING Huimin
LI Yanjie
QIN Hewei
HAO Chenyuan
Related Institution
Hubei Shizhen Laboratory
Hubei Provincial Institute of TCM,Hubei Key Laboratory of Theory and Application Research of Liver and Kidney in TCM
Hubei Provincial Hospital of Traditional Chinese Medicine(TCM)
The First Clinical Medical School,Hubei University of Chinese Medicine
Henan Provincial Hospital of Traditional Chinese Medicine