Dtermination of Tetramethylpyrazine in Fufang Chuanxiong Capules by HPLC
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Dtermination of Tetramethylpyrazine in Fufang Chuanxiong Capules by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 10, Issue 5, Pages: 1-3(2004)
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Published:2004
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LAN Shun. Dtermination of Tetramethylpyrazine in Fufang Chuanxiong Capules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(5): 1-3.
DOI:
LAN Shun. Dtermination of Tetramethylpyrazine in Fufang Chuanxiong Capules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(5): 1-3.DOI:
Dtermination of Tetramethylpyrazine in Fufang Chuanxiong Capules by HPLC
Objectivt:To establish a determining method for Tetramethylpyrazine(TMP)in Fufang Chuanxiong Capules by high-perfomance liquid chromatography.Methods:Tetramethylpyrazine was extracted from Fufang Chuanxiong Capules by 80% methanol.Nova-Pak C18 colum(3.9mm×300mm
4μm) was used as stationary phase
methanol-H 2O(50:50v/v)was used as mobile phase
the flow rate was 0.7mL·min-1 and the wavelength of detection was at 282nm.Results:The calibration curve was showed good linearity within the concentration range of 5~50 μg·mL-1 r=0.9996
the average recovery was 99.0%
RSD=1.2%.Conclusion:The established method is accurate