The Efficacy and the Mechanism of Tong Xin Luo on the Inhibition of Endothelial Cell Apoptosis Induced by Hypoxia

ZENG He-song; LIU Zheng-xiang; MA Ye-xin

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The Efficacy and the Mechanism of Tong Xin Luo on the Inhibition of Endothelial Cell Apoptosis Induced by Hypoxia

更新时间：2024-01-04

- The Efficacy and the Mechanism of Tong Xin Luo on the Inhibition of Endothelial Cell Apoptosis Induced by Hypoxia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 10, Issue 3, Pages: 27-30(2004)
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- Published：2004
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ZENG He-song, LIU Zheng-xiang, MA Ye-xin. The Efficacy and the Mechanism of Tong Xin Luo on the Inhibition of Endothelial Cell Apoptosis Induced by Hypoxia[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(3): 27-30.
DOI：

ZENG He-song, LIU Zheng-xiang, MA Ye-xin. The Efficacy and the Mechanism of Tong Xin Luo on the Inhibition of Endothelial Cell Apoptosis Induced by Hypoxia[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(3): 27-30. DOI：

摘要

目的:研究通心络对缺氧诱导的血管内皮细胞凋亡及Caspase-3活性的影响。方法:培养的牛主动脉内皮细胞分为三组:正常对照组

缺氧组和缺氧加通心络组

24h后检测凋亡细胞数并计算凋亡百分比

以及Caspase-3蛋白的表达与活性。结果:三组细胞凋亡百分比分别为7±0.12%

28.85±2.46%和11.48±1.4%(P<0.05);通心络对缺氧后血管内皮细胞的Caspase-3蛋白表达无影响

但可抑制其活性增高(41.2±5.5U/ml vs 67.8±7.2U/ml

P<0.05)。结论:通心络减少缺氧所致血管内皮细胞凋亡与抑制Caspase-3的活性有关。

Abstract

Objective:Determine the efficacy of Tong Xin Luo(TXL)on the apoptosis and the activity of Caspase-3 in the endothelial cell under hypoxia.Method:the Bovine aortic endothelial cells(BAEC) were divided into three groups and incubated with normal medium

hypoxia medium

hypoxia medium+TXL(50ug/ml)for 24h respectively.Apoptosis were measured with TUNEL.Western blot and the kit of Caspase-3 activity determination were used to measure the expression and the activity of Caspase-3 in BAEC.Result:TXL can decrease the percent of apoptotic cells in BAEC induced by hypoxia with mark significance (11.84±1.4% vs 28.85±2.46%

P<0.05).Although TXL had no effect on the expression of Caspase-3 after hypoxia stimulation

TXL could abolished the increase of the activity of Caspase-3 in BAEC induced by hypoxia(41.2±5.5 VS 67.8±7.2 U/ml

P<0.05).Conclusion:The inhibition of the activity of Caspase-3 might be the main mechanism of TXL decrease endothelial cell apoptosis induced by hypoxia.

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