Determination of Arginine in  Fort.Using HPLC with Evaporative Light-Scattering Detector(ELSD)

ZHAO Yu-xin; LI Man-ling

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Determination of Arginine in Fort.Using HPLC with Evaporative Light-Scattering Detector(ELSD)

更新时间：2024-01-04

- Determination of Arginine in Fort.Using HPLC with Evaporative Light-Scattering Detector(ELSD)
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 10, Issue 1, Pages: 8-9(2004)
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- Published：2004
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ZHAO Yu-xin, LI Man-ling. Determination of Arginine in Fort.Using HPLC with Evaporative Light-Scattering Detector(ELSD)[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(1): 8-9.
DOI：

ZHAO Yu-xin, LI Man-ling. Determination of Arginine in Fort.Using HPLC with Evaporative Light-Scattering Detector(ELSD)[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(1): 8-9. DOI：

摘要

目的:建立中药板蓝根中精氨酸的含量测定方法。方法:以Luna C18(4.6mm×150mm

5μm)色谱柱为固定相

柱温25℃;以水-三氟醋酸(99.8:0.2)为流动相

流速0.2ml·min-1;蒸发光散射检测器检测

漂移管温度105℃

气体流速3.00L·min-1。结果:精氨酸在0.504μg～2.52μg范围内线性关系良好

r=0.9992。平均加样回收率为99.3%

RSD=0.9%(n=5)。结论:该方法简便、准确

重复性好。

Abstract

Objective:To establish a method for the determination of arginine in Isatis indigotica Fort.Method:Chromatography was performed on a 4μm Luna C18 column (4.6mm×150mm)at 25℃.The mobile phase was composed of water-trifluoroacetic acid(99.8∶0.2)with a flow rate of 0.2ml5min-1.An Alltech-500 evaporative light-scattering detector(USA)was used with a gas flow rate of 3.00 L5min-1 and tube temperature of 105℃.Result:A good relationship between logarithm of peak area and logarithm of concentration was noted at 0.504μg～2.52μg.Average recovery of arginine was 99.30% and the RSD was 0.87%.Conclusion:It was proved that the method was reliable

simple and accurate.

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