Effect of Anti-fibrosis Compound Contained Serum on Matix Metalloproteinase and Tissue Inhibitor of Metalloprotenase-1 Gene Expression of HSC-LI90 Cells

ZHAO Gang; WANG Ling-tai; CHEN Jian-jie; ZHANG Bing

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Effect of Anti-fibrosis Compound Contained Serum on Matix Metalloproteinase and Tissue Inhibitor of Metalloprotenase-1 Gene Expression of HSC-LI90 Cells

更新时间：2024-01-04

- Effect of Anti-fibrosis Compound Contained Serum on Matix Metalloproteinase and Tissue Inhibitor of Metalloprotenase-1 Gene Expression of HSC-LI90 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 9, Issue 4, Pages: 31-33(2003)
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- Published：2003
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ZHAO Gang, WANG Ling-tai, CHEN Jian-jie, et al. Effect of Anti-fibrosis Compound Contained Serum on Matix Metalloproteinase and Tissue Inhibitor of Metalloprotenase-1 Gene Expression of HSC-LI90 Cells[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(4): 31-33.
DOI：

ZHAO Gang, WANG Ling-tai, CHEN Jian-jie, et al. Effect of Anti-fibrosis Compound Contained Serum on Matix Metalloproteinase and Tissue Inhibitor of Metalloprotenase-1 Gene Expression of HSC-LI90 Cells[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(4): 31-33. DOI：

摘要

目的:观察抗纤复方含药血清对肝星形细胞(HSC)LI90细胞(HSC-LI90)基质金属蛋白酶及其抑制因子基因表达的影响。方法:以0.5、2.0、4.0g/kg等不同剂量抗纤复方灌胃大鼠制备的含药血清

作用HSC-LI90细胞48h

应用Northern印迹杂交的方法

检测膜型基质金属蛋白酶、基质金属蛋白酶-2及其抑制因子-1(TIMP-1)基因的表达

并用酶图法检测基质金属蛋白酶-2的活性。结果:抗纤复方含药血清抑制HSC-LI90细胞TIMP-1

增加HSC-LI90细胞膜型基质金属蛋白酶基因表达

对基质金属蛋白酶-2基因表达及其活性没有影响。结论:抗纤复方抑制HSC-LI90细胞TIMP-1基因表达水平

促进胶原降解

可能是抗肝纤维化的作用机制之一。

Abstract

Objective:To observe the in vitro effect of anti-fibrosis Compound contained (AFC) serum on matix metalloproteinase and tissue inhibitor of metalloprotenase-1 (TIMP-1) mRNA levels of HSC-LI90 cell. Methods:HSC-LI90 cells were exposed in different doses of AFC contained serum(0.5

2.0 4.0g/kg) for 48 hours.The gene expressions of matix metalloproteinase-2(MMP-2) and membrane type matrix metalloproteinase (MT-MMP) and TIMP-1 were investigated by Northern blot

The gelatinase activity of MMP-2 was examined by zymography.Results:AFC contained serum could inhibit the TIMP-1 and increased the MT-MMP the gene expressions of HSC-LI90

The expressions of MMP-2 and MMP-2 activity were not affected by different doses AFC contained serum.Conclusions:AFC contained serum can decrease TIMP-1 mRNA expression of HSC-LI90 cells.

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