Determination of Arctiin in Bangboruexiao Granules by HPLC

WANG Zhao-hua; ZHANG Da-jun; ZHAO Yu-lan; XU Guo-jing

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Determination of Arctiin in Bangboruexiao Granules by HPLC

更新时间：2024-01-04

- Determination of Arctiin in Bangboruexiao Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 10, Issue 1, Pages: 10-11(2004)
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- Published：2004
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WANG Zhao-hua, ZHANG Da-jun, ZHAO Yu-lan, et al. Determination of Arctiin in Bangboruexiao Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(1): 10-11.
DOI：

WANG Zhao-hua, ZHANG Da-jun, ZHAO Yu-lan, et al. Determination of Arctiin in Bangboruexiao Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(1): 10-11. DOI：

摘要

目的:应用高效液相色谱法测定蒡勃乳蛾消颗粒中牛蒡苷的含量。方法:采用Diamonsil-C18柱(5μm

150mm×4.6mm)

乙睛-水(28:72)为流动相

检测波长280nm。结果:该方法的线性范围为0.2875～1.4375μg

r=0.9994

n=5

平均回收率为98.09%

RSD=0.73%。结论:本方法简便、准确、灵敏

可用于该制剂的质量控制。

Abstract

Objective:To determine the content of Arctiin in Bangboruexiao Granules by high performance liquid chromatography.Methods:The determination was conducted by HPLC using a Diamonsil-C18 column(5μm

150mm×4.6mm)and a mobile phase of acetonitrile-water(28∶72).The detection wavelength was 280nm.Results:Good linear relationship between area and amount was noted for 0.2875～1.4375μg of Arctiin with a correlation coefficient of 0.9994 and n was 5.The average recovery of Arctiin was 98.09%

RSD was 0.73%.Conclusion:The established method is simple

accurate

sensitive and can be applied to the quality control of the preparation.

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