The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR

XIE Chang-shang; ZHOU Wei-shun; FANG Zheng-quan; GUO Yong; WU Liang-cun

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The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR

更新时间：2024-01-04

- The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 9, Issue 4, Pages: 26-30(2003)
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- Published：2003
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XIE Chang-shang, ZHOU Wei-shun, FANG Zheng-quan, et al. The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(4): 26-30.
DOI：

XIE Chang-shang, ZHOU Wei-shun, FANG Zheng-quan, et al. The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(4): 26-30. DOI：

摘要

目的:研究复方中药——复方三根制剂对多药耐药(MDR)细胞株的逆转作用。方法:应用MTT比色法

观察复方三根制剂对MDR细胞的逆转作用。运用流式细胞仪(FCM)

测定其对耐药细胞积聚和外排阿霉素(ADR)的影响

以及对耐药株细胞表达pgp的影响。结果:复方三根制剂可部分恢复K562/ADR和K562/VCR耐药细胞对ADR的敏感性

而对VCR抗药性的逆转作用不明显;可增加K562/ADR和K562/VCR耐药细胞内ADR的积聚

并对外排ADR有一定影响;可部分下调p-gp的表达。结论:复方三根制剂可部分逆转耐药细胞的抗药性。

Abstract

Obsjective To observe the reversal effect of Fufang Sangen Mixture(FFSG) in multidrug resistant(MDR)cell lines K562/ADR and K562/VCR.Methods We used MTT assay to observe the mixture's reversal effect.Then

we determined the effect of FFSG on intracellular adriamycin(ADR) accumulation

drug efflux and the expression of p-gp by flowcytometry(FCM).Results 10mg/ml

20mg/ml of FFSG can reverse the K562/ADR cell's resistance to adriamycin to some degree and 20mg/ml FFSG can also partly reverse the K562/VCR cell's resistance to adriamycin.10mg/ml

20mg/ml concentration of FFSG can enhance the intracellular adriamycin accumulation in K562/ADR and K562/VCR cell lines.FFSG also has the effect on the efflux of intracellular adriamycin and decreasing the expression of p-gp in MDR cell lines.Conclusion FFSG can partly reverse the resistance of MDR cell lines.

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