Influence of Huang Lian Jie Du Decoction-Containing Serum on Neutrophil-Endothelial Cell Adhesion

FANG Su-ping; QIU Quan-ying; HAO Yu

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Influence of Huang Lian Jie Du Decoction-Containing Serum on Neutrophil-Endothelial Cell Adhesion

更新时间：2024-01-04

- Influence of Huang Lian Jie Du Decoction-Containing Serum on Neutrophil-Endothelial Cell Adhesion
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 7, Issue 3, Pages: 31-34(2001)
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- Published：2001
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FANG Su-ping, QIU Quan-ying, HAO Yu. Influence of Huang Lian Jie Du Decoction-Containing Serum on Neutrophil-Endothelial Cell Adhesion[J]. Chinese journal of experimental traditional medical formulae, 2001, 7(3): 31-34.
DOI：

FANG Su-ping, QIU Quan-ying, HAO Yu. Influence of Huang Lian Jie Du Decoction-Containing Serum on Neutrophil-Endothelial Cell Adhesion[J]. Chinese journal of experimental traditional medical formulae, 2001, 7(3): 31-34. DOI：

摘要

以体外培养的人脐静脉内皮细胞(HUVEC)为模型

应用MTT和蛋白染料染色法

观察给药一次及连续给药3d后0.5、1、1.5、2、2.5h所取黄连解毒汤(HLJDT)含药血清对HUVEC增殖及粘附中性粒细胞(PMN)能力的影响

以探讨HLJDT含药血清制备的最佳取血时间点及其抗炎作用机制。结果:①与对照血清相比

40%及20%浓度的含药血清对HUVEC均有不同程度的抑制增殖作用

而10%浓度时无明显抑制作用;②各点的HLJDT含药血清均有一定的抑制HUVECPMN粘附的作用

且与取血时间点存在一定的时效关系。其抑制细胞粘附的最佳时间点为连续给药3d组的1.5h左右。结论:HLJDT含药血清能抑制HUVEC-PMN粘附

可能是HLJDT抗炎作用的机制之一。

Abstract

In order to observe Huang Lian Jie Du Decoction (HLJDD)'s anti-inflammation mechanism and the best time to collect its herbage-containing serum from rats

human umbilical vein endothelial cells (HUVEC) cultured in vitro and protein staining was used to study the effect of serum on neutrophil-endothelial cell adhesion by treated with HUVEC for 4h、12h、24h

the serum was collected on 0.5h 1.0h 1.5h 2.0h 2.5h after treating with HLJDD (one dose once a day while another dose twice a day for three days).The results showed that the herbage-containing serum could decrease the cell adhesion in some degree with time-effect relationship.Compared to the control group

the best time to collect its serum was 1.5h around by dosed twice a day for three days.The HLJDD herbage-containing serum could decrease the neutrophil-endothelial cell adhesion

and inhibit neutrophil transuding from vessel

which might be one of HLJDD's anti-inflammation mechanisms.

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Related Institution

Chengdu University of Traditional Chinese Medicine（TCM）

Chongqing TCM Hospital

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Institute of Chinese Materia Medica,Academy of Chinese Medical Sciences

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