YU Jing, WANG Xiao-ping, CHEN Jun. Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of with Polyamide Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 23-26.
DOI:
YU Jing, WANG Xiao-ping, CHEN Jun. Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of with Polyamide Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 23-26. DOI: 10.11653/syfj2013080023.
Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of with Polyamide Resin
Objective: To optimize separation and purification technology of chlorogenic acid in leaves of Lonicera japonica. Method: With the content of chlorogenic acid as index
static and dynamic absorption
desorption properties of polyamide resin were observed by single factor test
and sample amount
the amount of washing water
type and dosage of eluent were investigated;With purity and retention rate as index
effects of elution flow rate
the concentration and volume of eluent on separation and purification technology was investigated by orthogonal test. Result: Optimized separation and purification technology was as following:sample amount 10 mg·g-1 resin
washed impurity with 2 BV water
and eluated with 3 BV the amount of 65% ethanol
flow rate of 0.4 BV·h-1
collected eluent. Conclusion: This optimized technology was stable and practicable
it could provide reference for industrial separation and purification of chlorogenic acid.
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Related Author
WANG Ping
LIANG Shaojia
ZHANG Wenwen
FENG Weihong
YI Hong
LI Chun
GUO Fengqian
SI Qin
Related Institution
Institute of Traditional Chinese Medicine(TCM),Shanghai University of TCM
Yanling DU Renkang Biotechnology Co. Ltd.
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Henan University of Chinese Medicine
Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province, Henan University of Chinese Medicine