Optimization of Extraction Technology for Total Flavonoids from  Seeds by Response Surface Methodolgy

CHEN Shi-qiang; LIN Jiang-xiao; CHEN Jian-feng; LIU Guo-min; GONH Lin-guang; LIANG Yi-chi

doi:10.11653/syfj2013080026

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Optimization of Extraction Technology for Total Flavonoids from Seeds by Response Surface Methodolgy

更新时间：2024-01-04

- Optimization of Extraction Technology for Total Flavonoids from Seeds by Response Surface Methodolgy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 8, Pages: 26-29(2013)
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- DOI：10.11653/syfj2013080026
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- Published：2013
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CHEN Shi-qiang, LIN Jiang-xiao, CHEN Jian-feng, et al. Optimization of Extraction Technology for Total Flavonoids from Seeds by Response Surface Methodolgy[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 26-29.
DOI：

CHEN Shi-qiang, LIN Jiang-xiao, CHEN Jian-feng, et al. Optimization of Extraction Technology for Total Flavonoids from Seeds by Response Surface Methodolgy[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 26-29. DOI： 10.11653/syfj2013080026.

摘要

目的: 优化油茶籽中总黄酮的乙醇提取工艺。方法: 以提取温度、提取时间、乙醇体积分数、料液比为自变量

应用Box-Behnken中心组合设计建立数学模型

以总黄酮提取率为响应值作响应面和等高线

采用响应面法优化油茶籽中总黄酮的提取工艺。结果: 最佳提取工艺条件为温度66 ℃

70%乙醇提取1.3 h

料液比1:22;油茶籽总黄酮提取率1.87%

与理论值(1.89%)接近。结论: 该优选工艺稳定可靠

为油茶资源的合理利用提供参考。

Abstract

Objective: To optimize extraction technology of total flavonoids from Camellia oleifera seeds. Method: With extraction temperature

extraction time

ethanol concentration and solid-liquid ratio as independent variables

Box-Behnken central composite design was used to establish mathematical model

response surface and contour were made with yield of total flavonoids as response value

response surface methodology was adopted to optimize extraction technology of total flavonoids from C. oleifera seeds. Result: Optimum extraction technology conditions were as followings:extraction temperature 66 ℃

ethanol concentration 70%

extracting time 1.3 h

solid-liquid ratio of 1:22.Under these conditions

yield of total flavonoids from C. oleifera seeds was 1.87%

which was close to the theoretical value of 1.89%. Conclusion: This optimized technology was stable and reliable

and it could provide reference for rational utilization of resources of C. oleifera.

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Related Institution

Maternal and Child Healthcare Hospital of Anji County in Zhejiang Province,Anji

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