HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus

MENG Xian-bo

doi:10.11653/syfj2013080077

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HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus

更新时间：2024-01-04

- HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 8, Pages: 77-79(2013)
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- DOI：10.11653/syfj2013080077
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- Published：2013
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MENG Xian-bo. HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 77-79.
DOI：

MENG Xian-bo. HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 77-79. DOI： 10.11653/syfj2013080077.

摘要

目的: 建立以高效液相色谱法测定凤眼草中槲皮素与山柰素含量的方法。方法: Inertsil ODS-3色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.4%磷酸溶液(50:50)

检测波长360 nm

流速1.0 mL·min-1。结果: 槲皮素在11.1～333 μg(r=1)线性关系良好

平均回收率为97.82%

RSD 1.1%;山柰素在2.3～69 μg (r=1)线性关系良好

平均回收率为96.81%

RSD 1.7%。结论: 方法简便、快速、重复性好

可用于凤眼草的质量控制。

Abstract

Objective: To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Ailanthi Fructus. Method: Quercetin and kaempferol were separated on Inertsil ODS-3 (4.6 mm×250 mm

5 μm) column and detected at 360 nm. The mobile phase was methanol-0.4% phosphate (50:50). The flow rate was 1.0 mL·min-1. Result: Quercetin and kaempferol were linear within the range of 11.1-333 μg (r=1)

2.3-69 μg (r=1) respectively. The average recovery was 97.82% with RSD of 1.1%

96.81% with RSD of 1.7% respectively. Conclusion: The method is simple

reproducible

and it can be used for the quality control of Ailanthi Fructus.

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Related Author

WEI Hua-ling

YANG Qing

WEI Hong-yin

LI Hui

XU Liang

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Related Institution

The First Clinical Medical College of Guangzhou University of Chinese Medicine

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences

The First Affiliated Hospital of Guangzhou University of Chinese Medicine

Level 3 Laboratory for State Administration of Traditional Chinese Medicine, Beijing Zhendong Gguangming Pharmaceutical Research Institute Co.Ltd.

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