Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive

QIU Xin-jian; ZHAO Hai-peng; LIU Xiang-dong; SU Rui-qiang; LI Shou-xin; ZHAO Zhi-quan

doi:10.11653/syfj2013080083

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Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive

更新时间：2024-01-04

- Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 8, Pages: 83-86(2013)
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- DOI：10.11653/syfj2013080083
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- Published：2013
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QIU Xin-jian, ZHAO Hai-peng, LIU Xiang-dong, et al. Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 83-86.
DOI：

QIU Xin-jian, ZHAO Hai-peng, LIU Xiang-dong, et al. Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 83-86. DOI： 10.11653/syfj2013080083.

摘要

目的: 考察不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分的影响。方法: 应用醇沉法、石硫法、石硫醇法、大孔树脂吸附法及萃取法等对金银花进行提取纯化

并采用高效液相色谱法测定其中6种咖啡酰奎宁酸类成分的含量。结果: 萃取法所得金银花提取物中咖啡酰奎宁酸类成分的含量最高

总咖啡酰奎宁酸含量达83.91%。结论: 萃取法是制备金银花提取物的理想方法。

Abstract

Objective: To study the effects of different purification ways on the extractive rate of caffeoyl quinic acids in Flos Lonicerae extractive. Method: Several purification processes were used respectively in the preparation of Flos Lonicerae extractive

such as alcohol precipitation method

lime-sulfur method

lime-sulfur ethanol method

macroporous resin method

and extraction method etc. The content of the six caffeoyl quinic acids was determined by HPLC. Result: The content of the caffeoyl quinic acids in Flos Lonicerae extractive by extraction method was higher than the other methods. Total content of caffeoyl quinic acids was up to 83.91%. Conclusion: The extraction method is the best in the preparation of Flos Lonicerae extractive.

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Related Institution

China Academy of Chinese Medical Sciences Orthopedics Institute

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