Comparative Study on the Component of Protein, Vitamin C, Vitamin E and Volatile Oil and SOD Activity of Fresh and Dried American Ginseng

ZHANG Jian-kui; GAO Rui; KANG Ting-guo; DOU De-qiang; WANG Gu-qiang

doi:10.11653/syfj2013080102

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Comparative Study on the Component of Protein, Vitamin C, Vitamin E and Volatile Oil and SOD Activity of Fresh and Dried American Ginseng

更新时间：2024-01-04

- Comparative Study on the Component of Protein, Vitamin C, Vitamin E and Volatile Oil and SOD Activity of Fresh and Dried American Ginseng
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 8, Pages: 102-106(2013)
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- DOI：10.11653/syfj2013080102
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- Published：2013
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ZHANG Jian-kui, GAO Rui, KANG Ting-guo, et al. Comparative Study on the Component of Protein, Vitamin C, Vitamin E and Volatile Oil and SOD Activity of Fresh and Dried American Ginseng[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 102-106.
DOI：

ZHANG Jian-kui, GAO Rui, KANG Ting-guo, et al. Comparative Study on the Component of Protein, Vitamin C, Vitamin E and Volatile Oil and SOD Activity of Fresh and Dried American Ginseng[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 102-106. DOI： 10.11653/syfj2013080102.

摘要

目的: 对西洋参鲜品和干品蛋白质、维生素C、维生素E、挥发油成分及超氧化物歧化酶活性进行比较

为西洋参的合理应用提供参考。方法: 蛋白质分析采用SDS-聚丙烯酰胺凝胶电泳法;维生素C、维生素E含量测定采用分光光度法;挥发油成分分析采用GC-MS的方法;超氧化物歧化酶(SOD)活性测定采用邻苯三酚自氧化法;并对测定结果进行t检验。结果: 西洋参鲜品与西洋参干品的蛋白质电泳谱带有所不同

但鲜品蛋白质含量高于干品;维生素C、维生素E含量、SOD活性西洋参鲜品极显著高于干品(P<0.01)

挥发油含量、成分种类西洋参鲜品多于干品

且成分有所不同。结论: 西洋参鲜品与干品的蛋白质、维生素C、维生素E、挥发油成分有所不同

SOD活性有所差异

这可能是二者药性及作用不同的原因之一。

Abstract

Objective: To compare the component of protein

vitamin C

vitamin E and volatile oil and superoside dismutase(SOD) activity of fresh and dried American ginseng and provide reference for reasonable application of American ginseng. Method: SDS-PAGE electrophoresis was carried out for protein analysis; UV spectrophotometry was used for determination of vitamin C and vitamin E; GC-MS was used for the analysis of volatile oil; SOD activity assay was detected with the methods of pyrogallol autoxidation and the data were analyzed by t-test. Result: The electrophoresis bands between fresh and dried American ginseng was different and the content of protein in fresh American ginseng was higher than dried one. The content of vitamin C and vitamin E

SOD activity assay in fresh American ginseng were significantly higher than dried one (P<0.01). The content and the kind of components of volatile oil were different and in fresh American ginseng was more than dried one. Conclusion: The component of protein

vitamin C

vitamin E

volatile oil

SOD activity in fresh American ginseng and dried one was different

which may be one of the reasons for the different of properties and efficacy.

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