LIU Wei, YANG Yan-ling, LIU Nai-qiang, et al. Study on Fingerprint and Determination of Saikosaponin b of Radix Bupleurioral Liquids by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 134-137.
DOI:
LIU Wei, YANG Yan-ling, LIU Nai-qiang, et al. Study on Fingerprint and Determination of Saikosaponin b of Radix Bupleurioral Liquids by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 134-137. DOI: 10.11653/syfj2013080134.
Study on Fingerprint and Determination of Saikosaponin b of Radix Bupleurioral Liquids by HPLC
Objective: To establish an HPLC method for a standard fingerprint and determination of saikosaponin b2 for quality control of Radix Bupleurioral liquids. Method: About 10 samples from different batches were analyzed by HPLC. By the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A)
mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the standard chromatogram was also calculated. Result: Eleven main marker peaks were selected in the standard fingerprint and 1 peak of them were identified. The similarity of all samples was over 0.985.The linear range of saikosaponin b2 was 0.039 7-0.793 3 μg (r=0.999 1). The average recovery was 98.27% with RSD of 1.79% (n=6). Conclusion: The developed method was reliable
and can be used for the quality control of Radix Bupleurioral liquids.