HPLC Method for Simultaneous Determination of 8 Kinds of Alkaloids in Total Alkaloids of Sophora Alopecuroides

LIU Zhen-long; Zhang Li; WEI Lei-chu; ZHENG Yan-xin; DENG Hong-zhu

doi:10.11653/syfj2013080142

您当前的位置：

首页 >

文章列表页 >

HPLC Method for Simultaneous Determination of 8 Kinds of Alkaloids in Total Alkaloids of Sophora Alopecuroides

更新时间：2024-01-04

- HPLC Method for Simultaneous Determination of 8 Kinds of Alkaloids in Total Alkaloids of Sophora Alopecuroides
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 8, Pages: 142-145(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013080142
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
LIU Zhen-long, Zhang Li, WEI Lei-chu, et al. HPLC Method for Simultaneous Determination of 8 Kinds of Alkaloids in Total Alkaloids of Sophora Alopecuroides[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 142-145.
DOI：

LIU Zhen-long, Zhang Li, WEI Lei-chu, et al. HPLC Method for Simultaneous Determination of 8 Kinds of Alkaloids in Total Alkaloids of Sophora Alopecuroides[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 142-145. DOI： 10.11653/syfj2013080142.

摘要

目的: 建立苦豆子总碱中生物碱的含量测定方法。方法: 采用HPLC

乙腈-0.05 mol·L-1磷酸二氢钾(三乙胺调pH>6.45)

梯度洗脱

柱温35 ℃

检测波长205 nm

流速1.0 mL·min-1。结果: 8种生物碱分离良好

8种生物碱含量>50%。结论: 该方法简便

可以作为苦豆子总碱的含量测定方法

比原标准中的滴定法测定总碱含量更方便、精确。

Abstract

Objective: To establish a method for the determination of alkaloids in the TASA. Method: HPLC method was used with acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate (triethylamine adjust pH value greater than 6.45) as mobile phase by gradient elution. The column temperature was kept at 35 ℃

and the detection wavelength was set at 205 nm. The flow rate was 1.0 mL·min-1. Result: Eight kinds of alkaloids were separated well

and the content was greater than 50%. Conclusion: The method is simple

more convenient and accurate for determination of the content of TASA than the original standard titration method.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quality Control of Salvianolic Acids Reference Extract

Studies on the Quality Standard of Toutongping Capsule

Study on Pharmacokinetics for TTS of Total Alkaloids of Sophora Flavecens Ait

Determination of Ferulic Acid in Pharagmitis Rhizoma Produced in Baiyangdian by UPLC

Comparison of Main Effective Constituent Content of in Chongqing Hou-shan and Other Domestic Areas

Related Author

Li-na LIU

Ying WANG

Hong-yu JIN

Hong-shui YUE

Shuang-cheng MA

Yao-lei LI

杜贵友

崔海峰

Related Institution

National Institutes for Food and Drug Control

Tianjin Tasly Pride Pharmaceutical Co.Ltd.

辽宁中医药大学

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰