Determination of Tetrodotoxin in Fugu Liver Using Hydrophilic Interaction Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

CHEN Xiao; WEN Hong-mei; LI Wei; ZHANG Lu; ZHAO Ji-hong

doi:10.11653/syfj2013080149

您当前的位置：

首页 >

文章列表页 >

Determination of Tetrodotoxin in Fugu Liver Using Hydrophilic Interaction Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

更新时间：2024-01-04

- Determination of Tetrodotoxin in Fugu Liver Using Hydrophilic Interaction Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 8, Pages: 149-152(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013080149
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
CHEN Xiao, WEN Hong-mei, LI Wei, et al. Determination of Tetrodotoxin in Fugu Liver Using Hydrophilic Interaction Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 149-152.
DOI：

CHEN Xiao, WEN Hong-mei, LI Wei, et al. Determination of Tetrodotoxin in Fugu Liver Using Hydrophilic Interaction Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 149-152. DOI： 10.11653/syfj2013080149.

摘要

目的: 建立河豚鱼肝中河豚毒素(TTX)的亲水液相色谱-三重四极杆质谱联用(LC-MS-MS)定量检测方法。方法: 样品经匀浆

超声

乙腈沉淀离心后

取上清液进样测定。极性聚酰胺键合硅胶柱InnovationTM HP Amide (100 mm×3.0 mm

5 μm)

流动相乙腈-0.3%甲酸(体积比为70:30)

流速为0.5 mL·min-1;柱温30 ℃

进样量5 μL。选择正离子多反应监测(MRM)模式

[M+H]+

m/z 320.1162.1进行定量分析。结果: TTX的线性范围为0.031 3～2.00 mg·L-1(r=0.999 9);检测限(S/N=3)为3.0 pg

加样回收率在96.3%～103.0%。结论: 该法灵敏度高

重复性好

简便快速

适用于河豚毒素的测定。

Abstract

Objective: To develop a LC-MS-MS method for determination of tetrodotoxin (TTX) in Fugu liver. Method: The analyte was vortex-mixed

extracted with acetonitrile by ultrasound

and followed by centrifugation. The supernatant (5 μL) was injected into the LC-MS-MS system for analysis. The separation was performed on an Innovation HP Amide column (100 mm×3.0 mm

5 μm) at 30 ℃ using acetonitrile-0.3% formic acid (70:30) as mobile phase at a flow rate of 0.5 mL·min-1. Quantification was through tandem-mass spectrometry with positive electro-spray ionization (ESI) and multiple reaction monitoring (MRM) at m/z [M+H]+320.1162.1 for TTX. Result: Calibration curve was linear over the TTX concentration range of 0.0313-2.00 mg·L-1 (r=0.999 9). The lower limit of quantification of TTX was 0.300 μg·L-1. The average recovery of TTX was 96.3%-103.0%. Conclusion: The validated method was shown to be simple

sensitive

rapid

reproducible and suitable for the determination of TTX in Fugu liver.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Wendantang in Intervention of ApoE^-/- Hyperlipidemic Mice Based on Liver Metabolomics

Effect of Jianpi Xiaoai Prescription on Expression of Metastasis-related Factors in Tumor Microenvironment of Nude Mouse Model of Liver Metastasis of Colon Cancer

Mechanism of Yuejuwan in Prevention and Treatment of Psychological and Heart Diseases Based on Liver TMT Labeled Quantitative Proteomics

Mori Folium Improves Glucose and Lipid Metabolism Disorders in Rats with Type 2 Diabetes Mellitus by Regulating PI3K/Akt/PPARα/CPT-1 Pathway

Effect of Suanzaoren Tang on Energy Metabolism of Liver Mitochondria in Aged Rats with Chronic Rapid Eye Movement Sleep Deprivation and Its Mechanism

Related Author

ZHOU Yun

YU Songren

ZHANG Lu

LIN Wenting

YU Keming

XIA Min

ZENG Zhijun

JI Yanhua

Related Institution

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory/Jiangxi Province Key Laboratory of Biological Etiologies of TCM/First-Level Discipline Platform of Chinese and Western Integrative Medicine/Experimental Animal and Scientific Technology Center of Jiangxi University of Chinese Medicine/School of Pharmacy， Jiangxi University of Chinese Medicine

Graduate School， Hunan University of Chinese Medicine

Wuhan Hospital of Traditional Chinese Medicine

Affiliated Hospital of Jiangxi University of Chinese Medicine

Affiliated Hospital of Hunan Academy of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰