LIU Jia-shui, LI Shi-jie, ZHANG Dan-yan, et al. Optimization of Extraction Technology for Total Alkaloids from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 41-44.
DOI:
LIU Jia-shui, LI Shi-jie, ZHANG Dan-yan, et al. Optimization of Extraction Technology for Total Alkaloids from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 41-44. DOI: 10.11653/syfj2013090041.
Optimization of Extraction Technology for Total Alkaloids from by Response Surface Methodology
摘要
目的: 优化南板蓝根中总生物碱的提取工艺。 方法: 在单因素试验基础上
选取提取时间、乙醇体积分数和液料比3个因素进行Box-Benhnken中心组合设计
以总生物碱提取率为因变量
利用响应曲面法对其提取工艺参数进行优化。 结果: 优选的提取工艺为提取时间20.23 min
乙醇体积分数83.21%
液料比21.16:1
南板蓝根总生物碱的最高理论提取率达0.781%。根据生产实际情况
将其修正为提取时间20.5 min
乙醇体积分数83.0%
液料比21:1
总生物碱提取率(0.78±0.024)%
与理论值较为接近。 结论: 优化的提取工艺准确可靠
具有一定的实用价值。
Abstract
Objective: To optimize extraction technology of total alkaloids from Baphicacanthus cusia. Method: Based on single factor test
effects of extraction time
ethanol concentration and liquid-solid ratio on yield of total alkaloids from B. cusia was investigated with a three-factor Box-Behnken center-united design
extraction parameters were optimized by response surface methodology. Result: Optimized extraction technology conditions was as following:extraction time 20.23 min
the concentration of ethanol 83.21 %
liquid-solid ratio 21.16: 1
theoretical yield of total alkaloids could be up to 0.781%.According to actual situation in production
optimum extraction parameters were modified as extraction time of 20.5 min
the concentration of ethanol 83.0%
liquid-solid ratio 21.0: 1
validated experiment showed yield of total alkaloids was (0.78 ± 0.024)% under these conditions
which was in consistency with the theoretic value. Conclusion: This modified extraction parameters were credible and reliable with some practical value.
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Related Author
LIU Jia-shui
LI Shi-jie
ZHANG Dan-yan
CHEN Yi-long
ZHENG Shao-yan
MENG Mei
WANG Hua-yu
REN Yu
Related Institution
School of Chinese Materia Medica,Guangzhou University of Chinese Medicine
Jiangxi University of Traditional Chinese Medicine
Key Laboratory of Active Ingredients in Traditional Chinese Medicine(TCM) Co-constructed by Jilin Province and Ministry of Education, Changchun University of Chinese Medicine
College of Pharmacy, Chengdu University of TCM
Ethnic Medicine School, Chengdu University of Traditional Chinese Medicine