Quality Standard of K-CoxB-JN

LI Zhi-hui; QIAN Yi; YE Jing; WEI Bao-hong; BAO Ju-tai; LIU Xiao-ya; CAO Yan-ling; WEI Ling-yu; ZHANG Yu-jie

doi:10.11653/syfj2013090073

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Quality Standard of K-CoxB-JN

更新时间：2024-09-23

- Quality Standard of K-CoxB-JN
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 9, Pages: 73-76(2013)
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- DOI：10.11653/syfj2013090073
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- Published：2013
- 稿件说明：
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LI Zhi-hui, QIAN Yi, YE Jing, et al. Quality Standard of K-CoxB-JN[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 73-76.
DOI：

LI Zhi-hui, QIAN Yi, YE Jing, et al. Quality Standard of K-CoxB-JN[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 73-76. DOI： 10.11653/syfj2013090073.

摘要

目的: 建立抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)定性和定量检测方法

为其提供质量控制手段。方法: 采用薄层色谱法对方中西洋参、麦冬、莱菔子、王不留行4味药物进行定性鉴别;采用HPLC-ELSD法测定西洋参中人参皂苷Rg1

Rb1含量。以 Agilent ZORBAX XDB C18(4.6 mm×250 mm

5 μm)为色谱柱

流动相乙腈-0.2%甲酸(梯度洗脱)

柱温为室温;ELSD参数:漂移管温度50℃

载气流速2.0 L·min-1

增益1。结果: 薄层色谱专属性强

分离度好

图谱斑点清晰

阴性对照无干扰;人参皂苷Rg1

Rb1质量分别在0.75~3.75

4.5~30

6~40 μg对数值与相应峰面积对数值呈良好的线性关系(r>0.999)

平均回收率分别为97.10%(RSD 3.96%)

95.15%(RSD 2.21%)

95.60%(RSD 3.00%)。结论: 该方法简便、准确、重复性好且无干扰

可用于K-CoxB-JN中主要药材的鉴别以及人参皂苷Rg1

Rb1的含量控制。

Abstract

Objective: To establish quantitative and qualitative determination for the quality control standard of K-CoxB-JN. Method: TLC was used to identify Panacis Quiaquefolii Radix

Ophiopogonis Radix

Raphani Semen and Vaccariae Semen. The content of ginsenoside Rg1

Rb1 in K-CoxB-JN was determined by HPLC-ELSD. An Agilent ZORBAX XDB C18 (4.6 mm×250 mm

5 μm) was used

and the mobile phase consisted of acetonitrile-0.2% formic acid (gradient elution). The temperature was set at room condition. The parameter of evaporative light-scattering detector: the temperature of drift tube was at 50℃ and the nebulizer nitrogen flow rate was 2.0 L·min-1; gain was 1. Result: The TLC spots were highly clear without the interference of negative sample

and had good separation effect. The linear relationship was obtained within the range of 0.75-3.75

4.5-30 and 6-40 μg for ginsenoside Rg1

Rb1 respectively (r>0.999). The average recoveries of the three components were 97.10% (RSD 3.96%)

95.15% (RSD 2.21%)

95.60% (RSD 3.00%) respectively. Conclusion: The method appeared to be simple

accurate

sensitive and reliable

and can be used in qualitative and quantitative analysis of K-CoxB-JN.

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Related Author

LI Zhi-hui1

QIAN Yi1

YE Jing1

WEI Bao-hong1

BAO Ju-tai2

LIU Xiao-ya1

CAO Yan-ling3

WEI Ling-yu1

Related Institution

School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine

College of Traditional Chinese Medicine,Hebei United University

School of Chinese Materia Medica,Haerbin University of Commerce,Haerbin

Guangzhou University of Chinese Medicine

Guangdong Institute of Traditional Chinese Medicine

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