YU Liang-zhong, WEN Ping, YU Jin-bao, et al. Determination of Luteolin in Caulis et Folium Stauntoniae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 111-113.
DOI:
YU Liang-zhong, WEN Ping, YU Jin-bao, et al. Determination of Luteolin in Caulis et Folium Stauntoniae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 111-113. DOI: 10.11653/syfj2013090111.
Determination of Luteolin in Caulis et Folium Stauntoniae by HPLC
摘要
目的: 建立野木瓜中木犀草素的含量测定方法。 方法: Dikma Diamonsil C18色谱柱(4.6 mm×250 mm
5 μm)
流动相乙睛-0.1%磷酸溶液(26:74)
流速 1 mL·min-1
检测波长350 nm
柱温室温。 结果: 线性范围0.067 75~2.168 μg(r=0.999 9)
平均回收率为99.25%
RSD 1.65%
精密度RSD 2.22%
重复性RSD 1.24%
稳定性RSD 2.58%。 结论: 方法稳定、可靠
可作为该药材的质量控制方法。
Abstract
Objective: To establish the method for determination of luteolin in Caulis et Folium Stauntoniae. Method: The chromatographic conditions was as follows: a Dikma Diamonsil C18 (4.6 mm×250 mm
5 μm) column
a mobile phase of acetonitrile-0.1% H3PO4 (26: 74); and a UV detector at 350 nm. Result: Luteolin was linear in the ranger of 0.067 75-2.168 μg (r=0.999 9); the average recovery of 99.25% (RSD 1.65%)
the precision with RSD 2.22%
repetition with RSD 1.24% and stability with RSD 2.58% were obtained. Conclusion: The method is accurate
reliable
and can be used for quality control of the production.