Objective: To establish a determination method of paeonol and paeoniflorin content in GFW and to compare its content discrepancy in GFW from different pharmaceutical factories. Method: HPLC was used to simultaneously determine the content of paeonol and paeoniflorin. An Elite Hypersil BDS C18(4.6 mm×250 mm

5 μm) column was used for content determination. The mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution. Flow rate was 1.0 mL·min-1. The detection wavelength was set at 230 nm (0-17 min)→274 nm (17-26 min). The injection volume was 20 μL and column temperature was maintained at 30℃. Result: The linear range of paeonol was 2.02-22.40 μg (r=0.999 9); the linear range of paeoniflorin was 2.40-20.26 μg (r=0.999 9). The average recovery was 99.5%

99.4%

and RSD was 0.96%

0.81% (n=9) respectively. Conclusion: The established chromatographic method which had good repeatability can be used to determine the content of paeonol and paeoniflorin in GFW simply and accurately and can be used to evaluate the quality of GFW from different pharmaceutical factories.