Objective: We explore a practical feed formulation in order to establish a stable atherosclerosis animal model.With high fat and hyperhomocysteinemia in shorter period. Method: Forty male SD rats were divided into 4 groups (n=10)

given different formula feeding with common feed

formulationⅠ(not containing propylthiouracil)

formulation II(containing propylthiouracil of 0.15%) and formulation Ⅲ(containing propylthiouracil of 0.25%). weighed once a week.The blood samples were collected at week 2 and 4 respectively

then to determine the levels of total cholesterol(TC)

low density lipoprotein cholesterin(LDL-C) in serum and homocysteine(Hcy) in plasma. Result: Compared with the control group

weight of formulationⅠhad no significant difference

while weight of formulation II and formulation Ⅲ decreased significantly (P<0.01);TC

LDL-C and Hcy levels in formulation II and formulation Ⅲ groups increased significantly (P<0.01) at 2

4 weeks

respectively

but formulationⅠhave no significant difference. Conclusion: In this study

we found that the rats fed with the formulation II can establish a stable atherosclerosis animal model with high fat and hyperhomocysteinemia in shorter period.