Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in

LI Yong-wei; WANG Chun-xia; BI Yong-yi

doi:10.11653/syfj2013090264

您当前的位置：

首页 >

文章列表页 >

Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in

更新时间：2024-09-23

- Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 9, Pages: 264-266(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013090264
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
LI Yong-wei, WANG Chun-xia, BI Yong-yi. Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 264-266.
DOI：

LI Yong-wei, WANG Chun-xia, BI Yong-yi. Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 264-266. DOI： 10.11653/syfj2013090264.

摘要

目的: 探讨黄连解毒汤对耐药性大肠杆菌逆转作用的影响。方法: 采用纸片法筛选产超广谱β-内酰胺酶大肠杆菌

确定黄连解毒汤的最低抑菌质量浓度

选取低于最低抑菌浓度的黄连解毒汤培养超广谱β-内酰胺酶大肠杆菌

确定最佳逆转效果的培养时间和药物浓度

并测定逆转率。结果: 黄连解毒汤对大肠杆菌ATCC8099最低抑菌浓度为0.6 g·L-1

逆转超广谱β-内酰胺酶大肠杆菌的最佳培养时间为30 h

最佳质量浓度为0.3 g·L-1

对氨苄西林药敏逆转率为60.0%、头孢他啶药敏逆转率为66.7%、头孢美唑药敏逆转率为75.0%

头孢曲松药敏逆转率为66.7%。结论: 黄连解毒汤对耐药性大肠杆菌逆转作用确切

可作为逆转耐药性大肠杆菌一种途径。

Abstract

Objective: To study the effect of Huanglian Jiedu Tang (HJT) in the reversal effect of drug resistance in Escherichia coli. Method: E. coli with extended-spectrum β-lactamase is screened by disc diffusion method. The minimum inhibitory concentration of HJT was determined.The extended-spectrum β-lactamase enzyme E. coli was cultured with a HJT concentration lower than the minimum inhibitory concentration. The cultivation duration and optimal concentration for reversal effect were selected

and the reversal rate was determined. Result: Minimum inhibitory concentration of HJT to the E. coli ATCC8099 was 0.6 g·L-1. The cultivation duration was 30 h and the optimal concentration for reversal effect was 0.3 g·L-1.The reversal rate of ampicillin was 60.0%. The reversal rate of ceftazidime was 66.7%. The reversal rate of cefmetazole was 75.0%. The reversal rate of ceftriaxone was 66.7%. Conclusion: The effect of HJT for reversal drug resistance in E. coli is defined. It is a way to reverse drug resistance in E. coli.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in Escherichia coli

Active Constituents and Mechanism of Epimedii Folium Against Liver Cancer： A Review

Overview of Experimental Methods for Antimicrobial Activity of Natural Drugs

Effect of Curcumin in Reversing Resistance of Human Colon Carcinoma Against Oxaliplatin Cell Line HCT-116/L-OHP

The Effect of Cholesterol Lowing and Anti-atherosclerosis of Hanglian Jiedu Tang and Its Components

Related Author

LI Yong-wei1

WANG Chun-xia1

BI Yong-yi2

Yan CHEN

Yuping LIU

Huangqin ZHANG

Yimin LIU

Xin-bo SONG

Related Institution

Department of Clinical Laboratory in Traditional Chinese Medicine,Hospital of Henan Province

Wuhan University School of Public Health

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine， Nanjing University of Chinese Medicine

Multi-component of Traditional Chinese Medicine （TCM） and Microecology Research Center， Jiangsu Province Academy of TCM

Tianjin University of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰