LIU Bin, WANG Xin-yu, SUN Xiao-xue, et al. Effect of Shenxiong Huayu Capsule Preconditioning on the Expression of VEGF after Cerebral Ischemia Reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 284-287.
DOI:
LIU Bin, WANG Xin-yu, SUN Xiao-xue, et al. Effect of Shenxiong Huayu Capsule Preconditioning on the Expression of VEGF after Cerebral Ischemia Reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 284-287. DOI: 10.11653/syfj2013090284.
Effect of Shenxiong Huayu Capsule Preconditioning on the Expression of VEGF after Cerebral Ischemia Reperfusion Injury in Rats
Objective: To observe the effect of Shenxiong Huayu capsule preconditioning on the expression of vascular endothelial growth factor(VEGF) after cerebral ischemia reperfusion injury in rats
to investigate the protective effect of shenxiong Huayu capsule on acute cerebral ischemia reperfusion injury in rats. Method: The rats were randomly divided into five groups:sham operatiion group
ischemia reperfusion group
Shenxiong Huayu capsule high
medium
low dose preconditioning group(480
240
120 mg·kg-1). The group were divided randomly into 3
6
12
24
48
72 h group after the 2 hour focal cerebral ischemia reperfusion(n=6). Intragastric administration 7 day
once a day. Middle cerebral artery occlusion (MCAO) and reperfushion model was made by an intraluminal filament method. The expressions of VEGF proteins were detected by using immunohistochemical method
the neurological deficit score was assessed at the same time. Result: Compared with the sham operation group
severe neurologic deficits of rats were presented and increases of VEGF in the ischemia reperfusion group at every time point(3
6
12
24
48
72 h)
and showing a significant difference(P<0.05
or P<0.01). Compared with the ischemia reperfusion group
neurologic deficits of rats were relieved significantly and VEGF expression increased distinctly in Shenxiong Huayu capsule dose preconditioning group at every time point(3
6
12
24
48
72 h)
showing a significant difference(P<0.05
or P<0.01)
high dose group showing best significant. Conclusion: Shenxiong Huayu Capsule may relive acute cerebral ischemia reperfusion injury in rats by increasing VEGF expression.
Effect of Shenxiong Huayu Capsule Preconditioning on the Expression of VEGF after Cerebral Ischemia Reperfusion Injury in Rats
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Related Author
LIU Bin
WANG Xin-yu
SUN Xiao-xue
DENG Chun-ying
CHEN Gui-hua
LIU Quan
HE Yiqing
ZHANG Ying
Related Institution
First Department of Neurology,Affiliated Hospital of Hebei United University
Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine
Hunan University of Chinese Medicine
MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials
Anhui Province Key Laboratory of Chinese Medicinal Formula